Colocalization of taurine- and cysteine sulfinic acid decarboxylase-like immunoreactivity in the hippocampus of the rat.
Colocalization of taurine- and cysteine sulfinic acid decarboxylase-like immunoreactivity in the hippocampus of the rat.
复制标题
大鼠海马中牛磺酸和半胱氨酸亚磺酸脱羧酶样免疫反应性的共定位。
DOI：
10.1002/syn.890040107
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Beitz,AJ
中科院分区：
文献类型：
--
作者：
Magnusson,KR;Clements,JR;Wu,JY;Beitz,AJ
有人提出牛磺酸是中枢神经系统中的抑制性神经递质/神经调节剂。本研究使用抗缀合牛磺酸 (Tau2) 的单克隆抗体结合抗半胱亚磺酸脱羧酶 (CSADC)（一种牛磺酸合成酶）的抗血清，定位大鼠海马内含牛磺酸的神经元。牛磺酸样免疫反应性 (Tau-LI) 和 CSADC-LI 共定位于齿状回、CA1(/CA2)、CA3 和 CA4 的神经元中。在所有检查的细胞中，齿状回颗粒细胞层内的锥体篮细胞被 Tau2 和 CSADC 染色最强烈。颗粒细胞也用 Tau-LI 和 CSADC-LI 双标记，CA1 区域的细胞核和树突的 Tau2 染色比体细胞更强烈。 CSADC-LI 与 Tau-LI 在这些神经元内共定位。 Tau2 和 CSADC 抗血清的浅染色在 CA3 和 CA4 神经元中的存在不一致，并且被发现高度依赖于固定类型和固定延迟。当多聚甲醛固定液中添加戊二醛时，Tau-LI 更一致地存在于 CA3 神经元数量增加中。延迟死后 0、6 或 24 小时后将海马浸泡在多聚甲醛中，在大多数研究的动物中，CA3 区域缺乏 Tau2 染色，类似于一些多聚甲醛灌注固定的大鼠。这些研究表明，牛磺酸存在于大鼠海马主要细胞层内的大多数神经元中，但在固定延迟后，Tau-LI 更容易从 CA3 区域的神经元中丢失。 Tau-LI 在兴奋性神经元（如颗粒细胞和锥体细胞）中的定位与其所提出的抑制性递质作用不一致。然而，CA1 区树突中显着的 Tau2 染色为以下假设提供了解剖学支持：牛磺酸可能从 CA1 区树突中释放出来，并可能充当这些锥体神经元中钙流的神经调节剂。
It is proposed that taurine is an inhibitory neurotransmitter/neuromodulator in the CNS. The present study localized taurine‐containing neurons within the rat hippocampus with the use of a monoclonal antibody against conjugated taurine (Tau2) in conjuction with an antiserum against cysteine sulfinic acid decarboxylase (CSADC), a synthesizing enzyme for taurine. Taurine‐like immunoreactivity (Tau‐LI) and CSADC‐LI were colocalized in neurons of the dentate gyrus, CA1(/CA2), CA3, and CA4. Of all the cells examined, pyramidal basket cells within the granule cell layer of the dentate gyrus were most intensely stained with both Tau2 and CSADC. Granule cells were also double‐labeled with Tau‐LI and CSADC‐LI Cell nuclei and dendrites in the CA1 region stained more intensely with Tau2 than somata. CSADC‐LI was colocalized with Tau‐LI within these neurons. Light staining with both Tau2 and the CSADC antiserum was inconsistently present in CA3 and CA4 neurons and was found to be highly dependent on the type of fixation and delay to fixation. Tau‐LI was more consistently present in increased numbers of neurons in CA3 when glutaraldehyde was added to the paraformaldehyde fixative solution. Hippocampi which were immersion‐fixed in paraformaldehyde following a 0‐, 6‐, or 24‐hour postmortem delay exhibited a lack of Tau2 staining in the CA3 region in the majority of animals studied, similar to some paraformaldehyde perfusion‐fixed rats. These studies suggest that taurine was present in the majority of neurons within the major cell layers of the rat hippocampus, but Tau‐LI was more easily lost from neurons in the CA3 region following delay to fixation. The localization of Tau‐LI in excitatory neurons such as granule cells and pyramidal cells is not consistent with its proposed inhibitory transmitter role. However, the prominent Tau2 staining in dendrites of the CA1 region provides anatomical support for the hypothesis that taurine may be released from dendrites in the CA1 region and may function as a neuromodulator of calcium flux in these pyramidal neurons.