Gene and cluster-specific expression of the Iroquois family members during mouse development
Gene and cluster-specific expression of the Iroquois family members during mouse development
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DOI：
10.1016/s0925-4773(01)00451-8
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发表时间：
2001-09-01
影响因子：
2.6
通讯作者：
Christoffels, VM
中科院分区：
文献类型：
--
作者：
Houweling, AC;Dildrop, R;Christoffels, VM
最近发现了果蝇易洛魁同源盒基因复合体的哺乳动物同源基因，参与分化的模式和区划(Mech.Dev.，69(1997)169；Dev.生物，217(2000)266；Dev.动力，218(2000)160；机械。Dev.，91(2000)317；Dev.生物，224(2000)263；基因组研究，10(2000)1453；机械。发展，103(2001)193)。小鼠家族的六个成员被发现组织在两个同源簇中，每个簇有三个基因，分别是irx1，-2，-4和irx3，-5，-6(Peter等人)。2000)。作为进一步研究它们的调控和功能的基础，我们对所有六个irx基因的基因组结构和表达模式进行了比较分析。这些基因在外胚层、中胚层和内胚层来源的组织中表达具有高度特异性和地区性。在大多数组织中，IRX1和IRX2以及IRX3和IRX5基因的表达模式非常相似，而IRX4和IRX6基因的表达模式则非常不同。有趣的是，同源基因在四肢、心脏和喉咽部邻近和相互作用的组织中的表达是相互排斥的。结果表明，Irx基因在簇水平上是协同调控的。(C)2001爱思唯尔爱尔兰科学有限公司。保留所有权利。
Mammalian homologues of the Drosophila Iroquois homeobox gene complex, involved in patterning and regionalization of differentiation, have recently been identified (Mech. Dev., 69 (1997) 169; Dev. Biol., 217 (2000) 266; Dev. Dyn., 218 (2000) 160; Mech. Dev., 91 (2000) 317; Dev. Biol., 224 (2000) 263; Genome Res., 10 (2000) 1453; Mech. Dev., 103 (2001) 193). The six members of the murine family were found to be organized in two cognate clusters of three genes each, Irx1, -2, -4 and Irx3, -5, -6, respectively (Peters et al.. 2000). As a basis for further study of their regulation and function we performed a comparative analysis of the genomic organization and of the expression patterns of all six Irx genes. The genes are expressed in highly specific and regionalized patterns of ectoderm, mesoderm and endoderm derived tissues. In most tissues the pattern of expression of the clustered genes, especially of Irx1 and -2 and of Irx3 and -5, respectively, closely resembled each other while those of Irx4 and -6 were very divergent. Interestingly, the expression of cognate genes was found to be mutually exclusive in adjacent and interacting tissues of limb, heart and the laryncho-pharyncheal region. The results indicate that the Irx genes are coordinately regulated at the level of the cluster. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.