Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor
Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor
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DOI：
10.1101/gad.1086803
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发表时间：
2003-07-01
影响因子：
10.5
通讯作者：
Hirose, S
中科院分区：
文献类型：
--
作者：
Shimojima, T;Okada, M;Hirose, S
染色质结构在转录调控中起着关键作用。果蝇Gaga因子引导染色质重塑到其结合部位。我们在这里证明了果蝇FACT(促进染色质转录)是dSPT16和dSSRP1的异源二聚体，通过其dSSRP1亚基与GagA因子结合，与核小体结合，促进GagA因子导向的染色质重塑。此外，编码GagA因子的三胸类基因与spt16之间的遗传相互作用导致了GagA因子-事实复合体在Hox基因表达中的作用。染色质免疫沉淀实验表明，GagA因子-FACT复合体存在于超级胸腔和腹壁B细胞的调节区。这些数据说明了事实在调节染色质结构以调节基因表达方面的关键作用。
Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates chromatin transcription), a heterodimer of dSPT16 and dSSRP1, is associated with GAGA factor through its dSSRP1 subunit, binds to a nucleosome, and facilitates GAGA factor-directed chromatin remodeling. Moreover, genetic interactions between Trithorax-like encoding GAGA factor and spt16 implicate the GAGA factor-FACT complex in expression of Hox genes Ultrabithorax, Sex combs reduced, and Abdominal-B. Chromatin immunoprecipitation experiments indicated the presence of the GAGA factor-FACT complex in the regulatory regions of Ultrabithorax and Abdominal-B. These data illustrate a crucial role of FACT in the modulation of chromatin structure for the regulation of gene expression.