Quantification of reactive oxygen species generation by photoexcitation of PEGylated quantum dots.
Quantification of reactive oxygen species generation by photoexcitation of PEGylated quantum dots.
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DOI：
10.1002/smll.201401209
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发表时间：
2014-12
期刊：
影响因子：
13.3
通讯作者：
E. Yaghini;K. Pirker;C. W. Kay;A. Seifalian;A. MacRobert
中科院分区：
文献类型：
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作者：
E. Yaghini;K. Pirker;C. W. Kay;A. Seifalian;A. MacRobert
利用量子点 (QD) 光催化产生活性氧 (ROS) 已被广泛报道，但缺乏对 ROS 形成及其量子产率的定量研究。本研究研究了具有红光发射的水溶性聚乙二醇化 CdSe/ZnS 量子点产生 ROS。量子点的聚乙二醇化通常用于赋予水溶性并最大限度地减少网状内皮系统器官的吸收；因此，ROS 形成的研究具有生物医学意义。使用非光解可见波长激发，超氧阴离子自由基被证明是产生的主要 ROS 物种，量子效率为 0.35%。通过原位照明的耗氧量测量和电子顺磁共振波谱法证明，在电子供体烟酰胺腺嘌呤二核苷酸 (NADH) 的存在下，产率可以显着提高。仅从量子点无法检测到单线态氧的直接产生。与由相同的量子点与磺化酞菁络合产生的 ROS 进行了比较，磺化酞菁可以通过量子点和酞菁之间的福斯特共振能量转移产生单线态氧。
Photocatalytic generation of reactive oxygen species (ROS) from quantum dots (QDs) has been widely reported yet quantitative studies of ROS formation and their quantum yields are lacking. This study investigates the generation of ROS by water soluble PEGylated CdSe/ZnS QDs with red emission. PEGylation of QDs is commonly used to confer water solubility and minimise uptake by organs of the reticuloendothelial system; therefore studies of ROS formation are of biomedical relevance. Using non-photolytic visible wavelength excitation, the superoxide anion radical is shown to be the primary ROS species generated with a quantum efficiency of 0.35%. The yield can be significantly enhanced in the presence of the electron donor, nicotinamide adenine dinucleotide (NADH), as demonstrated by oxygen consumption measurements and electron paramagnetic resonance spectroscopy with in situ illumination. Direct production of singlet oxygen is not detectable from the QDs alone. A comparison is made with ROS generation by the same QDs complexed with a sulfonated phthalocyanine which can generate singlet oxygen via Förster resonance energy transfer between the QDs and the phthalocyanine.