COATING OF PROTEINS TO POLYSTYRENE ELISA PLATES IN THE PRESENCE OF DETERGENTS
COATING OF PROTEINS TO POLYSTYRENE ELISA PLATES IN THE PRESENCE OF DETERGENTS
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DOI：
10.1016/0022-1759(88)90205-0
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发表时间：
1988-02-10
影响因子：
2.2
通讯作者：
LEWARTOWSKA, A
中科院分区：
文献类型：
--
作者：
GARDAS, A;LEWARTOWSKA, A
研究了几种清洗剂对聚苯板蛋白质涂层的影响。当浓度等于或高于各自的临界胶束浓度(CMC)时，所有洗涤剂都会阻止蛋白质附着到聚苯乙烯上。然而，在浓度低于其cmc值10-100倍的洗涤剂存在的情况下，可以在聚苯乙烯平板上实现蛋白质涂层。CMC与将蛋白质与聚苯乙烯结合抑制50%的洗涤剂浓度有很强的相关性。甲状腺质膜抗原结合率的等级相关系数为0.97(P<；0.01)。洗涤剂对塑料表面蛋白质涂层效率的影响也在一定程度上取决于蛋白质与洗涤剂的相互作用。与疏水的甲状腺质膜抗原或甲状腺球蛋白相比，洗涤剂对小分子亲水性蛋白(如人免疫球蛋白)附着的抑制作用更强。一些洗涤剂不会干扰塑料上的蛋白质附着，并允许随后进行疏水性、洗涤剂增溶的质膜蛋白质的ELISA法检测。
The influence of several detergents on protein coating of polystyrene plates has been investigated. At concentrations equal to or higher than their respective critical micelle concentration (CMC) all detergents prevent attachment of proteins to polystyrene. However, coating of proteins on polystyrene plates can be achieved in the presence of detergents at concentrations 10-100 times lower than their CMC values. There is a strong correlation between the CMC and the detergent concentration inhibiting protein binding to polystyrene by 50%. The rank correlation coefficient for the binding of thyroid plasma membrane antigens was 0.97 (P < 0.01). The influence of a detergent on the efficiency of protein coating of plastic surfaces also depends to some extent on protein-detergent interactions. Attachment of small hydrophilic proteins such as human IgG is more strongly inhibited by detergents than is the coating of hydrophobic thyroid plasma membrane antigens or thyroglobulin. Some detergents do not interfere with protein attachment to plastics and permit subsequent ELISA assays for hydrophobic, detergent solubilized plasma membrane proteins.