Polymorphisms of the α-synuclein promoter:: expression analyses and association studies in Parkinson's disease
Polymorphisms of the α-synuclein promoter:: expression analyses and association studies in Parkinson's disease
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DOI：
10.1007/s00702-002-0769-5
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发表时间：
2003-01-01
影响因子：
3.3
通讯作者：
Riess, O
中科院分区：
文献类型：
--
作者：
Holzmann, C;Krüger, R;Riess, O
Mutations of the a-synuclein gene have shown to be relevant in some rare families with autosomal dominant Parkinson's disease (PD). Furthermore, a-synuclein protein is a major component of the Lewy bodies also in sporadic PD patients. Increased levels of wildtype a-synuclein in the cell leads to increased intracellular hydrogen peroxide levels and causes death of dopaminergic neurons in rat primary culture. Subsequently, oxidative stress has been directly linked with a-synuclein aggregation in vitro. This raises the question whether increased cc-synuclein expression might be linked to higher susceptibility to PD and whether alpha-synuclein promoter polymorphisms are associated with PD: Here, two polymorphisms (-116C>G and -668T>C) of the alpha-synuclein promoter defining four haplotypes have been characterized in 315 German PD patients. The influence of the four haplotypes on gene expression was studied by CAT reporter gene assays in neuronal SK-N-AS cells. The -668C/-116G haplotype revealed significant higher CAT expression than the -668T/-116G or the -668T/-116C haplotype, respectively. Although the -668C/-116G haplotype was more common in PD patients, this difference was not significant.
