Presence of Activated Airway T Lymphocytes in Human Puumala Hantavirus Disease
Presence of Activated Airway T Lymphocytes in Human Puumala Hantavirus Disease
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DOI：
10.1378/chest.10-2791
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发表时间：
2011-09-01
期刊：
影响因子：
9.6
通讯作者：
Ahlm, Clas
中科院分区：
文献类型：
--
作者：
Rasmuson, Johan;Pourazar, Jamshid;Ahlm, Clas
背景：汉坦病毒引起两种临床综合征：肾综合征出血热（HFRS）和汉坦病毒肺综合征（HPS）。HFRS的临床症状通常还包括呼吸道症状。由于对HFRS肺部发病机制的了解有限，我们旨在进一步研究下气道局部气道免疫反应。方法：对15例住院HFRS患者行支气管镜检查，支气管内粘膜活检及BAL液取样。活组织切片染色检测白细胞、淋巴细胞亚群和血管内皮粘附分子。流式细胞术测定BAL液和血淋巴细胞亚群。14名健康志愿者作为对照组。结果：与对照组相比，HFRS患者支气管黏膜活检显示上皮和粘膜下层CD8(+) T细胞数量增加(P
Background: Hantaviruses cause two clinical syndromes: hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). The clinical spectrum in HFRS also often involves respiratory symptoms. As information about the pulmonary pathogenesis in HFRS is limited, we aimed to further study the local airway immune response in the lower airways.Methods: Bronchoscopy was performed in 15 hospitalized patients with HFRS, with sampling of endobronchial mucosal biopsies and BAL fluid. Biopsies were stained for leukocytes, lymphocyte subsets, and vascular endothelial adhesion molecules. BAL fluid and blood lymphocyte subsets were determined using flow cytometry. Fourteen healthy volunteers acted as a control group.Results: Compared with control subjects, endobronchial mucosal biopsies from patients with HFRS revealed increased numbers of CD8(+) T cells in both epithelium and submucosa (P