The nitrogen assimilation control (Nac) protein represses asnC and asnA transcription in Escherichia coli
The nitrogen assimilation control (Nac) protein represses asnC and asnA transcription in Escherichia coli
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DOI：
10.1111/j.1574-6968.2002.tb11001.x
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发表时间：
2002-01-10
影响因子：
2.1
通讯作者：
Camarena, L
中科院分区：
文献类型：
--
作者：
Poggio, S;Domeinzain, C;Camarena, L
在这项工作中，我们表明，asnA和asnC基因的表达是由生长介质中的铵的可用性调节。我们的研究结果表明，在氮限制生长条件下，氮同化控制（Nac）蛋白参与asnC基因的阻遏，其产物是激活asnA转录所必需的。我们还表明，天冬酰胺对asnA的表达产生负面影响，独立于Nac的存在。这些结果使我们能够得出结论，asnA的转录是由两种不同的机制，响应于不同的效应：氮和天冬酰胺的可用性。(C)2002年由Elsevier Science B.V.代表欧洲微生物学会联合会出版。
In this work, we show that the expression of the asnA and asnC genes is regulated by the availability of ammonium in the growth medium. Our results suggest that, under nitrogen-limiting growth conditions, the nitrogen assimilation control (Nac) protein is involved in the repression of the asnC gene, whose product is required to activate the transcription of asnA. We also show that asparagine negatively affects the expression of asnA, independently of the presence of Nac. These results allow us to conclude that asnA transcription is regulated by two different mechanisms that respond to different effectors: nitrogen and asparagine availability. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.