The mouse small ubiquitin-like modifier-2 (SUMO-2) inhibits interleukin-12 (IL-12) production in mature dendritic cells by blocking the translocation of the p65 subunit of NFκB into the nucleus
The mouse small ubiquitin-like modifier-2 (SUMO-2) inhibits interleukin-12 (IL-12) production in mature dendritic cells by blocking the translocation of the p65 subunit of NFκB into the nucleus
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DOI：
10.1016/j.molimm.2011.05.002
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发表时间：
2011-09-01
影响因子：
3.6
通讯作者：
Chun, Taehoon
中科院分区：
文献类型：
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作者：
Kim, Eun Mi;Lee, Han-Hyoung;Chun, Taehoon
小泛素样修饰物（SUMO）的翻译后修饰参与了几个重要的细胞事件。特别地，I κ B α的SUMO-1和SUMO-4修饰已显示积极参与NF κ B调节。然而，在SUMO家族中，SUMO-2/3的具体功能仍然相对未知。此外，尚不清楚SUMO-2/3在NF κ B活化过程中是否遵循与SUMO-1和SUMO-4相同的功能作用。在这项研究中，我们研究了小鼠SUMO-2在树突状细胞（DC）成熟过程中的影响。我们的结果表明SUMO-2的异位表达不影响细胞表面MHC II类分子（A（B））和共刺激分子（CD 80和CD 86）的表达以及抗原摄取效率。然而，小鼠SUMO-2的异位表达通过阻断NF κ B B的p65亚单位向细胞核的移位而抑制IL-12的分泌，这导致初始CD 4（+）T细胞向辅助性T细胞2（Th 2）偏移的极化。进一步的分析表明SUMO-2直接修饰I κ B α。这些结果表明SUMO-2/3在调节NF κ B活性中的功能作用在进化过程中是保守的。(C)2011爱思唯尔有限公司保留所有权利。
Post-translational modification by small ubiquitin-like modifier (SUMO) is involved in several significant cellular events. In particular, SUMO-1 and SUMO-4 modifications of I kappa B alpha have been shown to be actively involved in NF kappa B regulation. However, among the SUMO family, the specific function of SUMO-2/3 remains relatively unknown. In addition, it is not clear whether SUMO-2/3 follows the same functional role as SUMO-1 and SUMO-4 during the activation of NF kappa B. In this study, we examined the influence of mouse SUMO-2 during the maturation of dendritic cells (DCs). Our results showed that the ectopic expression of SUMO-2 does not affect the cell surface expression of MHC class II molecule (A(b)) and co-stimulatory molecules (CD80 and CD86), and the efficiency of antigen uptake. However, the ectopic expression of mouse SUMO-2 inhibited IL-12 secretion by blocking the translocation of the p65 subunit of NF kappa B into the nucleus, which led to the polarization of naive CD4(+) T cells to T helper 2 (Th2) shift in vitro. Further analyses showed that SUMO-2 directly modified I kappa B alpha. These results indicate that the functional role of SUMO-2/3 in the regulation of NF kappa B activity was conserved during evolution. (C) 2011 Elsevier Ltd. All rights reserved.