NUCLEASE DIGESTION OF DNA AND RNA IN NUCLEI FROM YOUNG-ADULT AND SENESCENT CAENORHABDITIS-ELEGANS (NEMATODA)
NUCLEASE DIGESTION OF DNA AND RNA IN NUCLEI FROM YOUNG-ADULT AND SENESCENT CAENORHABDITIS-ELEGANS (NEMATODA)
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DOI：
10.1016/0047-6374(86)90102-8
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发表时间：
1986-03-01
影响因子：
5.3
通讯作者：
VANFLETEREN, JR
中科院分区：
文献类型：
--
作者：
MEHEUS, L;VANFLETEREN, JR
用微球菌核酸酶和DNA酶Ⅰ消化从幼线虫和衰老线虫中提取的细胞核。微球菌核酸酶消化细胞核的动力学没有随年龄的变化。然而，有一个与年龄相关的增加，由DNA酶I释放的酸溶性脱氧核糖核苷酸，这表明染色质构象的微妙变化发生在老年线虫。随着蠕虫龄的增长，核RNA与DNA的比例下降，核RNA变得更容易被酶降解。这些发现似乎表明，在老线虫中，核RNA受到蛋白质的保护较少。随着年龄的增长，核RNA/DNA比率的下降与普遍接受的观点一致，即老年动物的RNA和蛋白质合成水平降低。
Nuclei prepared from young adult and senescent Caenorhabditis elegans (Nematoda) were subjecte to digestion by micrococcal nuclease and DNase I. The kinetics of digestion of nuclei by micrococcal nuclease showed no change with age. There was, however, an age-related increase of acid-soluble deoxyribonucleotides released by DNase I, suggesting that subtle alterations of chromatin conformation occur in aged nematodes. The ratio of nuclear RNA to DNA decreased and the nuclear RNA became more susceptible to enzymatic degradation as the worms grew old. These findings appear to indicate that nuclear RNA is less protected by protein in old nematodes. The decline of the nuclear RNA/DNA ratio with age is in good agreement with the generally accepted idea that there is a reduced level of RNA and protein synthesis in old animals.