Remodeling of suspended small intestinal submucosa venous valve:: An experimental study in sheep to assess the host cells' origin
Remodeling of suspended small intestinal submucosa venous valve:: An experimental study in sheep to assess the host cells' origin
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DOI：
10.1097/01.rvi.0000058410.01661.62
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发表时间：
2003-03-01
影响因子：
2.9
通讯作者：
Rösch, J
中科院分区：
文献类型：
--
作者：
Brountzos, E;Pavcnik, D;Rösch, J
目得：为探讨小肠粘膜下层（SIS）方形支架型二尖瓣（VVs）重建过程中宿主细胞的来源，材料与方法：将VVs悬置在方形裸支架内，使瓣膜元件在展开后不与静脉壁接触，从而形成悬置VVs。将8个SVV放置在4只成年雌性绵羊的肝内和肝下下腔静脉（IVC）内。在这些动物的颈外静脉中植入8个标准VV。对比放置5周后，检查器械的稳定性和通畅性，并处死动物。大体，组织学和扫描电子显微镜（SEM）examinations.RESULTS：随访现场X线片和静脉造影显示没有迁移的设备，静脉闭塞，或血栓形成。所有SVV均完好无损，未与IVC壁接触。6个VV合格，2个略微倾斜，有一些返流。组织学研究显示SVV和VV重塑，新形成胶原纤维;发现成纤维细胞和炎性细胞穿透SIS瓣叶和表面上的内皮细胞。还存在SIS新生血管形成。SVV和VV瓣叶游离部分之间的SIS重塑无差异。VV瓣叶基部较游离瓣叶基部厚（P <0.01）。扫描电镜下可见SVV和VV两侧的内皮细胞。SVV中心瓣叶表面和VV瓣叶的两个表面的内皮化比SVV leafles.CONCLUSION的外周表面更完整：SIS为基础的瓣膜重塑发生独立的血管壁接触的细胞直接从循环的招聘。
PURPOSE: To investigate the origin of host cells during remodeling of small intestinal submucosa (SIS) square stent-based bicuspid venous valves (VVs).MATERIALS AND METHODS: Suspended VVs (SVVs) were developed by suspending VVs within bare square stents so the valve elements would not contact the vein wall after deployment. Eight SVVs were placed within the intrahepatic and infrahepatic inferior venae cavae (IVCs) of four adult female sheep. Eight standard VVs were implanted in the external jugular veins of these animals for. comparison. At 5 weeks after placement, the devices were examined for stability and patency and the animals were killed. Gross, histologic, and scanning electron microscopic (SEM) examinations were performed.RESULTS: Follow-up spot radiographs and venography showed no migration of the devices, venous occlusion, or thrombus formation. All SVVs were intact without contact with the IVC wall. Six VVs were competent and two were slightly tilted with some reflux. Histologic study showed remodeling of SVVs and VVs with newly formed collagen fibers; fibroblasts and inflammatory cells were found penetrating the SIS leaflets and endothelial cells on the surface. SIS neovascularization was also present. There was no difference regarding SIS remodeling between SVVs and the free part of VV leaflets. The VV leaflets' bases were thicker compared to their free parts (P < .01). SEM examination showed endothelial cells on both sides of the SVVs and VVs. Endothelialization of the SVV central leaflet surfaces and both surfaces of the VV leaflets was more complete than that of the peripheral surfaces of the SVV leaflets.CONCLUSION: SIS-based valve remodeling occurs independently of vessel wall contact by recruitment of cells directly from the circulation.