Dehydroepiandrosterone protects tissues of streptozotocin-treated rats against oxidative stress
Dehydroepiandrosterone protects tissues of streptozotocin-treated rats against oxidative stress
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DOI：
10.1016/s0891-5849(99)00012-x
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发表时间：
1999-06-01
影响因子：
7.4
通讯作者：
Boccuzzi, G
中科院分区：
文献类型：
--
作者：
Aragno, M;Tamagno, E;Boccuzzi, G
糖尿病中的慢性高血糖决定了自由基的过量产生，并且越来越多的证据表明自由基会导致糖尿病并发症的发生。最近有报道称，脱氢表雄酮具有抗氧化特性；这项研究评估了每天一次口服给药，持续三周，是否可以为链脲佐菌素诱导的糖尿病大鼠的组织提供抗氧化保护。对糖尿病动物的肝脏、大脑和肾脏匀浆进行脂质过氧化评估，测量硫代巴比妥酸反应物质和荧光色素的稳态浓度。高血糖大鼠的硫代巴比妥酸反应物质形成和荧光色素脂质水平高于对照组。脱氢表雄酮治疗（4 毫克/天，持续 3 周）保护组织免受脂质过氧化：脱氢表雄酮治疗动物的肝脏、肾脏和脑匀浆显示硫代巴比妥酸反应物质和荧光色素形成显着减少。还研究了脱氢表雄酮对细胞抗氧化防御的影响，因为抗氧化酶活性受损被认为是氧依赖性毒性的证据。在肾和肝匀浆中，脱氢表雄酮治疗使还原型谷胱甘肽的胞质水平以及超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶的酶活性恢复到接近对照值。在大脑中，仅过氧化氢酶活性明显增加（p < .05），但通过脱氢表雄酮治疗可恢复。结果表明，DHEA 治疗明显减少了链脲佐菌素治疗大鼠组织中的氧化应激产物。 (C) 1999 爱思唯尔科学公司。
Chronic hyperglycemia in diabetes determines the overproduction of free radicals, and evidence is increasing that these contribute to the development of diabetic complications. It has recently been reported that dehydroepiandrosterone possesses antioxidant properties; this study evaluates whether, administered daily for three weeks per os, it may provide antioxidant protection in tissues of rats with streptozotocin-induced diabetes. Lipid peroxidation was evaluated on liver, brain and kidney homogenates from diabetic animals, measuring both steady-state concentrations of thiobarbituric acid reactive substances and fluorescent chromolipids. Hyperglycemic rats had higher thiobarbituric acid reactive substances formation and fluorescent chromolipids levels than controls. Dehydroepiandrosterone-treatment (4 mg/day for 3 weeks) protected tissues against lipid peroxidation: liver, kidney and brain homogenates from dehydroepiandrosterone-treated animals showed a significant decrease of both thiobarbituric acid reactive substances and fluorescent chromolipids formation. The effect of dehydroepiandrosterone on the cellular antioxidant defenses was also investigated, as impaired antioxidant enzyme activities were considered proof of oxygen-dependent toxicity. Tn kidney and liver homogenates, dehydroepiandrosterone treatment restored to near-control values the cytosolic level of reduced glutathione, as well as the enzymatic activities of superoxide-dismutase, glutathione-peroxidase, catalase. In the brain, only an increase of catalase activity was evident (p < .05), which reverted with dehydroepiandrosterone treatment. The results demonstrate that DHEA treatment clearly reduces oxidative stress products in the tissues of streptozotocin-treated rats. (C) 1999 Elsevier Science Inc.