23Na NMR demonstrates prolonged increase of intracellular sodium following transient regional ischemia in the in situ pig heart.
23Na NMR demonstrates prolonged increase of intracellular sodium following transient regional ischemia in the in situ pig heart.
复制标题
23Na NMR 显示原位猪心脏短暂局部缺血后细胞内钠的长期增加。
DOI：
10.1007/s003950050127
复制
发表时间：
1999
影响因子：
9.5
通讯作者：
Balschi,JA
中科院分区：
文献类型：
--
作者：
Balschi,JA
This study tests the hypothesis that Na+iincreases during regional ischemia in thein situpig heart.An extracorporeal shunt was created between the carotid artery and the left anterior descending artery of 14 open chest pigs.23Na and31P NMR spectroscopy measured myocardial Na+iand high energy phosphates (HEPs). The protocol consisted of three 40 min periods: pre-ischemia (shunt pressure, 76±23 mmHg (S.D.)), ischemia (shunt pressure, 25±7 mmHg), and post-ischemia (shunt pressure, 53±11 mmHg). The pre-ischemia Na+iconcentration was 6.7±4.2 mM. Phosphocreatine (PCr) was 15.3±0.5 mM, ATP 9.4±0.4 mM, inorganic phosphate (Pi) 1.5±0.2 mM, and pHi7.16±0.09. At the end of ischemia Na+ihad increased to 10.5±2.8 mM (p<0.0002); PCr decreased to 5.9±2.1 mM (p<0.0002); ATP was 6.5±0.5 mM (p<0.003); Pi had increased to 6.3±1.0 mM (p<0.0002), and pHiwas 6.41±0.06 (p<0.0002). During the first 10 min of the reperfusion, Na+iincreased further to 12.4±2.8 mM (p<0.025), whereas HEPs all returned to pre-ischemic values.Na+iincreases during regional ischemia in thein situpig heart, suggesting reduced Na+/K+ATPase activity. While ATP probably does not limit Na+/K+ATPase activity, increases in Pi and decreases in pHimay reduce Na+/K+ATPase activity. Additional Na+iincreases during reperfusion suggest either augmented Na+influx or decreased Na+efflux.
