Interactions between phosphatidylcholines and cholesterol in monolayers at the air/water interface
Interactions between phosphatidylcholines and cholesterol in monolayers at the air/water interface
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DOI：
10.1016/j.colsurfb.2004.06.007
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发表时间：
2004-08-15
影响因子：
5.8
通讯作者：
Hac-Wydro, K
中科院分区：
文献类型：
--
作者：
Dynarowicz-Latka, P;Hac-Wydro, K
胆固醇和合成磷脂的混合物，不同的饱和度的磷脂酰胆碱（PC）的酰基链，如二硬脂酰磷脂酰胆碱（DSPC），硬脂酰-油酰磷脂酰胆碱（SOPC）和二油酰磷脂酰胆碱（DOPC）已被研究作为浮动的Langmuir单分子膜在空气/水界面。为了检查极性基团的影响，选择二硬脂酰磷脂酰乙醇胺（DSPE）。将膜在室温下铺展在含水亚相上，并通过表面压力-面积（pi-A）等温线和压缩模量（C-s（-1））值来表征。通过分析平均分子面积来检查相互作用，并通过混合值的过量自由能来量化。所得到的结果表明，胆固醇饱和/不饱和磷脂酰胆碱的亲和力没有显着差异，并揭示了强烈的影响的极性基团的种类上的胆固醇-磷脂相互作用。另一方面，非极性基团结构被发现修改甾醇-PC复合物的化学计量。(C)2004 Elsevier B. V.保留所有权利。
Mixtures of cholesterol and synthetic phospholipids, differing in saturation of phosphatidylcholine (PC) acyl chains, such as distearoyl phosphatidylcholine (DSPC), stearoyl-oleoyl phosphatidylcholine (SOPC) and dioleoyl phosphatidylcholine (DOPC) have been studied as floating Langmuir monolayers at the air/water interface. In order to examine the influence of a polar group, distearoyl phosphatidyl ethanolamine (DSPE) was chosen. The films were spread at room temperature on aqueous subphases and characterized by the surface pressure-area (pi-A) isotherms and compression modulus (C-s(-1)) values. The interactions were examined by analyzing the mean molecular areas and quantified by the excess free energy of mixing values. The obtained results indicate that the affinity of cholesterol to saturated/unsaturated phosphatidylcholines does not differ significantly, and revealed strong influence of the kind of a polar group on the cholesterol-phospholipid interactions. On the other hand, the apolar group structure was found to modify the stoichiometry of sterol-PC complexes. (C) 2004 Elsevier B.V. All rights reserved.