The vacuolar amino acid transport system is a novel, direct target of GATA transcription factors
The vacuolar amino acid transport system is a novel, direct target of GATA transcription factors
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液泡氨基酸转运系统是 GATA 转录因子的新型直接靶点
DOI：
10.1093/bbb/zbaa041
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Sekito Takayuki
中科院分区：
文献类型：
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作者：
Sato Akane;Kimura Takumi;Hondo Kana;Kawano-Kawada Miyuki;Sekito Takayuki
在酿酒酵母中，Avt 4从液泡中输出中性和碱性氨基酸。先前的研究表明，加塔转录因子Gln 3和Gat 1参与了AVT 4的转录，它们是调节细胞对氨基酸状态变化做出反应的关键调节因子。在此，我们发现AVT 4启动子的GATA结合位点的突变降低了AVT 4的表达，同时，染色质免疫沉淀（ChIP）分析显示Gat 1-Myc 13与AVT 4启动子结合。先前的微阵列结果证实，gln 3-gat 1-gln 3细胞显示AVT 1和AVT 7的表达减少，AVT 1和AVT 7也编码空泡氨基酸转运蛋白。此外，ChIP分析显示，编码液泡酸性氨基酸输出蛋白的AVT 6是加塔转录因子的一个新的直接靶点。加塔转录因子对AVT转运蛋白表达的广泛影响表明液泡氨基酸转运整合到细胞氨基酸稳态中。
InSaccharomyces cerevisiae, Avt4 exports neutral and basic amino acids from vacuoles. Previous studies have suggested that the GATA transcription factors, Gln3 and Gat1, which are key regulators that adapt cells in response to changes in amino acid status, are involved in theAVT4transcription. Here, we show that mutations in the putative GATA-binding sites of theAVT4promoter reducedAVT4expression.Consistently, a chromatin immunoprecipitation (ChIP) assay revealed that Gat1-Myc13binds to theAVT4promoter. Previous microarray results were confirmed thatgln3∆gat1∆ cells showed a decrease in expression ofAVT1andAVT7, which also encode vacuolar amino acid transporters. Additionally, ChIP analysis revealed that theAVT6encoding vacuolar acidic amino acid exporter represents a new direct target of the GATA transcription factor. The broad effect of the GATA transcription factors on the expression of AVT transporters suggests that vacuolar amino acid transport is integrated into cellular amino acid homeostasis.