Developmental regulation of activated ERK expression in the spinal cord and dorsal root ganglion of the chick embryo
Developmental regulation of activated ERK expression in the spinal cord and dorsal root ganglion of the chick embryo
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DOI：
10.1016/j.neures.2005.01.001
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发表时间：
2005-05-01
影响因子：
2.9
通讯作者：
Shiga, T
中科院分区：
文献类型：
--
作者：
Kato, T;Ohtani-Kaneko, R;Shiga, T
丝裂原活化蛋白激酶（MAPK）参与响应各种细胞外信号的细胞内途径。细胞外信号调节激酶（ERK）是MAPKs的一员，在神经发育中具有多种功能。然而，在体内分布的活化形式的ERK（p-ERK）在发育中的神经系统还没有得到很好的理解。本实验研究了p-ERK在鸡胚脊髓和背根神经节（DRG）中的表达。在脊髓中，p-ERK阳性细胞出现在腹侧脑室区胚胎第4天（E4）。从E6开始，它们出现在灰质和白色物质中，表明从脑室区迁移。双标记法显示，这些p-ERK阳性细胞包括少突胶质细胞前体。在背角，p-ERK阳性小细胞出现在E6。随后，在背角的阳性细胞数量短暂增加，然后显着减少E10。运动神经元在ET时也有p-ERK的瞬时表达。在DRG，E5时腹外侧区出现弱的p-ERK免疫反应。从E6开始，免疫反应性变得更强，到E9，在整个DRG中观察到强烈的p-ERK阳性细胞。这些数据为开始研究ERK在神经发育中的体内作用提供了神经解剖学框架。(c)2005年Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
Mitogen-activated protein kinases (MAPKs) are involved in the intracellular pathways that respond to various extracellular signals. Extracellular signal-regulated kinase (ERK) is a member of MAPKs and has various functions in neural development. However, the in vivo distribution of the activated form of ERK (p-ERK) in the developing nervous system is not well understood. Here, we investigated the expression of p-ERK in the spinal cord and dorsal root ganglion (DRG) of chick embryos. In the spinal cord, p-ERK-positive cells appeared in the ventral ventricular zone on embryonic day 4 (E4). From E6 onward, they appeared in the gray matter and in the white matter, suggesting migration from the ventricular zone. A double labeling method revealed that these p-ERK-positive cells included oligodendrocyte precursors. In the dorsal horn, p-ERK-positive small cells appeared on E6. Subsequently, the positive cells in the dorsal horn increased transiently in number and then decreased markedly by E10. Motoneurons also expressed p-ERK transiently on ET In the DRG, weak p-ERK immunoreaction appeared in the ventrolateral region on E5. From E6, the immunoreactivity became stronger and by E9 intense p-ERK-positive cells were observed throughout the DRG. These data provide a neuroanatomical framework to begin to examine the in vivo role of ERK in neural development. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.