62Cu-PTSM and PET used for the assessment of angiotensin II-induced blood flow changes in patients with colorectal liver metastases
62Cu-PTSM and PET used for the assessment of angiotensin II-induced blood flow changes in patients with colorectal liver metastases
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62Cu-PTSM和PET用于评估血管紧张素II引起的结直肠肝转移患者的血流变化
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
T. Allen
中科院分区：
文献类型：
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作者：
M. Flower;J. Zweit;A. Hall;D. Burke;M. Davies;M. Dworkin;H. Young;J. Mundy;R. Ott;V. McCready;P. Carnochan;T. Allen
摘要。本研究的目的是建立一种定量正电子发射断层扫描（PET）方法来研究血管紧张素II （AII）诱导的肝脏血流分布的变化。这是为了评估血管操作在结肠直肠癌肝转移患者局部化疗中的作用。所选择的示踪剂是铜-62 （II）丙酮醛双-（n4 -甲基）硫代氨基脲（62Cu-PTSM），该示踪剂在动脉内给药后具有较高的首过萃取率和组织保留率。示踪剂的半衰期短，且其可从62Zn/62Cu发生器获得，因此可以在一次成像过程中对患者进行短间隔重复PET扫描。示踪剂在肝脏内的分布在一个单一的视图成像使用PET相机旋转大面积探测器。通过优化采集方案，可以获得足够的数据来产生高质量的图像，并以≤10%的精度量化示踪剂摄取。对一名连续三次获得62Cu-PTSM PET扫描的患者进行了成像方法的重复性评估，该患者未进行血管操作。对9名患者进行了一系列扫描（在45分钟AII输注之前、期间和之后），以评估与长时间血管操作相关的血流变化。在大多数病例（7/11个病变；7/9例患者）中观察到显著的个体反应，在流量变化的大小和持续时间上都有所不同。这些发现说明了62Cu-PTSM和PET在药理学研究中的潜力。个体患者对AII输注的广泛反应表明，PET血流评估对于选择该程序可能有效的患者具有价值。
Abstract. The aim of this study was to establish a quantitative positron emission tomography (PET) method for investigating angiotensin II (AII)-induced changes in blood flow distribution in the liver. This was in order to evaluate the role of vascular manipulation applied to locoregional chemotherapy treatment in patients with colorectal liver metastases. The tracer selected was copper-62 (II) pyruvaldehyde bis-(N4-methyl)thiosemicarbazone (62Cu-PTSM), which exhibits high first-pass extraction and tissue retention following intra-arterial administration. The short half-life of the tracer and its availability from a 62Zn/62Cu generator enabled short-interval repeat PET scans on patients in a single imaging session. Distribution of tracer within the liver was imaged in a single view using a PET camera with rotating large-area detectors. By optimisation of the acquisition protocol, it was possible to acquire sufficient data to produce good-quality images and to quantify tracer uptake with an accuracy of ≤10%. Reproducibility of the imaging method was assessed in a single patient in whom three consecutive 62Cu-PTSM PET scans were obtained, and in whom no vascular manipulation was performed. Sets of scans (before, during and immediately after a 45-min AII infusion) were obtained in nine patients to assess blood flow changes associated with prolonged vascular manipulation. Significant individual responses, varying in both the magnitude and the duration of flow change, were observed in the majority of cases (7/11 lesions; 7/9 patients). These findings illustrate the potential of 62Cu-PTSM and PET for pharmacological studies. The wide range of individual patient responses to AII infusion suggests that PET blood flow assessment would be of value for selecting patients in whom this procedure may be effective.