Interpreting gelatinase activity in tumor tissue and serum as a prognostic marker of naturally developing canine tumors.
Interpreting gelatinase activity in tumor tissue and serum as a prognostic marker of naturally developing canine tumors.
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将肿瘤组织和血清中的明胶酶活性解释为自然发展的犬肿瘤的预后标志物。
DOI：
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发表时间：
2005
影响因子：
1.2
通讯作者：
H. Sakamoto
中科院分区：
文献类型：
--
作者：
Kanako Miya;K. Misumi;N. Miyoshi;K. Arai;M. Fujiki;C. Kubota;H. Sakamoto
为了评价组织和血清明胶酶活性作为犬肿瘤预后标志物的临床实用性，在活检和手术时收集来自60个肿瘤的组织样品和来自相同动物的相应血清样品。根据组织病理学和临床侵袭性将病例分为6类：非炎性（Inf（-））和炎性（Inf（+））良性、Inf（-）MR（-）、Inf（-）MR（+）、Inf（+）MR（-）和Inf（+）MR（+）恶性。明胶酶活性测定半定量使用明胶酶谱与明胶酶标准培养犬外周血单核细胞刺激脂多糖。组织提取物中明胶酶活性在良恶性肿瘤之间无显著差异。Inf（+）良性肿瘤以及Inf（-）MR（+）、Inf（+）MR（-）和Inf（+）MR（+）恶性肿瘤的组织明胶酶活性均显著高于Inf（-）良性肿瘤。Inf（-）MR（-）恶性组的组织活性显著低于Inf（+）MR（-）和Inf（+）MR（+）恶性组。恶性肿瘤患者血清中的活性明显高于正常对照组和良性肿瘤患者。Inf（-）MR（+）、Inf（+）MR（-）和Inf（+）MR（+）恶性肿瘤诱导的血清明胶酶活性显著高于Inf（-）良性肿瘤。血清中明胶酶活性与肿瘤提取物中明胶酶活性呈正相关。肿瘤组织和血清中明胶酶的升高可能与炎症以及肿瘤的侵袭性相关，因此应结合组织病理学用于预测肿瘤转移或复发。
To evaluate the clinical usefulness of tissue and serum gelatinase activity as a prognostic marker of canine tumors, tissue samples from 60 tumors and corresponding serum samples from the same animals were collected at the time of biopsy and surgery. On the basis of histopathology and clinical aggressiveness of metastasis and recurrence (MR), the cases were divided into 6 categories: non-inflammatory (Inf(-)) and inflammatory (Inf(+)) benign, and the Inf(-) MR(-), Inf(-) MR(+), Inf(+) MR(-), and Inf(+) MR(+) malignant. Gelatinase activity was determined semi-quantitatively using gelatin zymogram with a gelatinase standard from cultured canine peripheral blood mononuclear cells stimulated with lipopolysaccharide. No significant difference in gelatinase activities in tissue extracts was evident between the benign and malignant tumors. Inf(+) benign tumors, as well as Inf(-) MR(+), Inf(+) MR(-) and Inf(+) MR(+) malignant tumors, showed significantly higher tissue gelatinase activity than Inf(-) benign. The tissue activity in Inf(-) MR(-) malignant was significantly lower than in Inf(+) MR(-) and Inf(+) MR(+) malignant. The serum activity was significantly higher in the malignant cases than in the control and the benign. Inf(-) MR(+), Inf(+) MR(-) and Inf(+) MR(+) malignant tumors induced significantly higher gelatinase activity in serum than Inf(-) benign tumors. Gelatinase activity in serum was positively correlated with that in tumor extracts. Increased gelatinase in tumor tissue and serum may be correlated with inflammation as well as tumor aggressiveness, and thus should be used in combination with histopathology for predicting tumor metastasis or recurrence.
影响因子：
15.9
作者：
Fang, KC;Raymond, WW;Caughey, GH
通讯作者：
Caughey, GH
从狗的健康关节和骨关节炎关节获得的滑液和滑膜中的明胶酶活性。
影响因子：
1
作者：
Volk,SusanW;Kapatkin,AmyS;Haskins,MarkE;Walton,RaquelM;D'Angelo,Marina
通讯作者：
D'Angelo,Marina
影响因子：
19.6
作者：
Rao, VH;Lees, GE;Cosgrove, D
通讯作者：
Cosgrove, D