Effects of bradykinin on lymphatic pumping in rat mesentery.
Effects of bradykinin on lymphatic pumping in rat mesentery.
复制标题
缓激肽对大鼠肠系膜淋巴泵的影响。
DOI：
10.1152/ajpgi.1996.270.5.g752
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Benoit,JN
中科院分区：
文献类型：
--
作者：
Yokoyama,S;Benoit,JN
研究了缓激肽对大鼠肠系膜集合管淋巴泵活性的影响，并评估了负责缓激肽反应的受体亚型。用腹腔内α-氯醛糖和乌拉坦麻醉大鼠，并使用活体显微镜技术研究外置的肠系膜。连续监测收集淋巴管的直径（约100微米），并计算淋巴泵参数（舒张末期直径、收缩末期直径、每搏输出量指数、射血分数、收缩频率和泵流量指数）。缓激肽（0.1-1.0 nM）不影响舒张末期内径、收缩末期内径、每搏输出量指数和射血分数。缓激肽以剂量依赖性方式增加淋巴管收缩频率和泵流量指数。Des-Arg 9-[Leu 8]缓激肽（B1拮抗剂，0.1 μ M）对基线淋巴泵没有影响，但完全抑制缓激肽诱导的收缩频率增加。N-乙酰-D-Arg-[Hyp 3，Thi 5，8，D-Phe 7]缓激肽（B2拮抗剂，0.1 μ M）在基线条件下显著抑制淋巴收缩频率，但对缓激肽诱导的收缩频率增加无影响。这些结果表明，缓激肽诱导积极的变时性，但不是通过刺激B1受体对淋巴泵活动的变力作用。
The effects of bradykinin on lymphatic pump activity of rat mesenteric collecting duct were studied, and the receptor subtype responsible for the bradykinin response was evaluated. Rats were anesthetized with intraperitoneal alpha-chloralose and urethan, and exteriorized mesenteries were studied using intravital microscopic techniques. The diameter of the collecting lymph vessels (approximately 100 microns) was continuously monitored and lymphatic pump parameters (end diastolic diameter, end systolic diameter, stroke volume index, ejection fraction, contraction frequency, and pump flow index) were calculated. Bradykinin (0.1-1.0 nM) did not affect end diastolic diameter, end systolic diameter, stroke volume index, and ejection fraction. Bradykinin increased lymphatic contraction frequency and pump flow index in a dose-dependent manner. Des-Arg9-[Leu8]bradykinin (B1 antagonist, 0.1 microM) had no effect on baseline lymphatic pumping but completely inhibited the bradykinin-induced increase in contraction frequency. N-acetyl-D-Arg-[Hyp3,Thi5,8,D-Phe7] bradykinin (B2 antagonist, 0.1 microM) significantly depressed lymphatic contraction frequency in baseline conditions but had no effect on bradykinin-induced increases in contraction frequency. These results indicate that bradykinin induces positive chronotropic but not inotropic effects on lymphatic pump activity through the stimulation of B1 receptors.