Identification of a cell-surface antigen selectively expressed on the natural killer cell
Identification of a cell-surface antigen selectively expressed on the natural killer cell
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自然杀伤细胞上选择性表达的细胞表面抗原的鉴定
DOI：
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发表时间：
1977
影响因子：
15.3
通讯作者：
H. Cantor
中科院分区：
文献类型：
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作者：
L. Glimcher;F. Shen;H. Cantor
我们研究了NZB和B6小鼠自然杀伤（NK）细胞的细胞表面表型，这些细胞对MuLV+淋巴肿瘤有反应。(a)NK细胞不表达Thy 1、Ly 2或IG表面标志物。(b)NK细胞表达被C3 H抗CE抗血清（“抗Ly1.2抗血清”）识别的抗原。由于B6和B6/Ly1.1同源株脾细胞的NK活性对C3 H抗CE抗血清同样敏感，因此NK抗原与Ly1.2不同。这一点通过以下观察得到证实：通过用来自B6或B6/Ly1.1同源菌株的脾细胞吸收C3 H抗CE抗血清，去除了α NK活性。用BALB/c胸腺细胞和脾细胞（其为Ly1.2+NK-）吸收C3 H α CE血清去除了抗Ly1.2活性并保持抗NK活性不变。该吸收步骤可以通过将BALB/c基因型插入到对CE细胞免疫的受体中来规避（即，(C3H X BALB/c）F1 α CE脾细胞）。这种抗血清，暂时称为“抗NK”，定义了一个新的淋巴细胞亚类，可能在肿瘤的免疫监视中发挥重要作用。
We have studied the cell-surface phenotype of natural killer (NK) cells of NZB and B6 mice which react to an MuLV+ lymphoid tumor. (a) NK cells do not express Thy1, Ly2, or Ig surface markers. (b) NK cells express an antigen recognized by C3H anti-CE antiserum ('anti-Ly1.2 antiserum'). Inasmuch as NK activity of spleen cells from B6 and B6/Ly1.1 congenic strains were both equally sensitive to C3H anti-CE antiserum, the NK antigen is distinct from Ly1.2. This point was confirmed by the observation that alphaNK activity was removed by absorption of C3H anti-CE antiserum with spleen cells from either B6 or B6/Ly1.1 congenic strains. Absorption of C3H alphaCE serum with BALB/c thymocytes and spleen cells (which are Ly1.2+NK-) removed anti-Ly1.2 activity and left anti-NK activity intact. This absorption step could be circumvented by inserting the BALB/c genotype into the recipient immunized to CE cells (i.e., (C3H X BALB/c)F1 alphaCE spleen cells). This antiserum, provisionally termed 'anti-NK', defines a new subclass of lymphocytes which may play a central role in the immunosurveillance against tumors.