Effect of 5-azaC on the growth, flowering time and sexual phenotype of spinach
Effect of 5-azaC on the growth, flowering time and sexual phenotype of spinach
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5-azaC对菠菜生长、开花时间及性表型的影响
DOI：
10.1134/s1021443715050118
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发表时间：
2015-09-01
影响因子：
1.4
通讯作者：
Gao, W. J.
中科院分区：
文献类型：
--
作者：
Li, S. F.;Zhang, G. J.;Gao, W. J.
菠菜（Spinacia oleracea L.）是一种二倍体雌异株植物，具有一对同态性染色体X和y。植物DNA甲基化是一种已知的基因组表观遗传修饰过程，可调节植物基因表达。为了探讨DNA甲基化对菠菜生长和性发育的影响，用去甲基化试剂5-azaC处理菠菜种子。研究结果包括发芽率、根长、株高、开花时间和性表型。结果表明，低浓度（30µM） 5-azaC对菠菜发育影响不大，但对种子萌发有促进作用。100 ~ 1000µM 5-azaC的发芽率、根长、株高与5-azaC呈负相关。5-azaC处理显著缩短了开花时间。此外，5-azaC影响了菠菜的性表型，显著增加了雌雄同株个体的百分比。这些结果可能表明，营养生长和生殖生长都受DNA甲基化的表观遗传调控。
Spinach (Spinacia oleracea L.) is a diploid dioecious plant with a pair of homomorphic sex chromosomes X and Y. Plant DNA methylation, a known process for genome epigenetic modification, regulates gene expression in plants. To explore the effects of DNA methylation on spinach growth and sexual development, spinach seeds were treated with the demethylating reagent 5-azaC. The resulting phenotypes were then investigated, including germination percentage, root length, plant height, flowering time, and sexual phenotype. Results showed that 5-azaC at a low concentration (30 µM) only slightly influenced spinach development but promoted seed germination. The germination percentage, root length, and plant height negatively correlated with 5-azaC at 100–1000 µM. The flowering time significantly reduced at all four treatments with 5-azaC. In addition, 5-azaC influenced the sexual phenotype of spinach and remarkably increased the percentage of monoecious individuals. These results may suggest that vegetative and reproductive growth are both epigenetically regulated by DNA methylation.