Impact of Goreisan components on rat mesenteric collecting lymphatic vessel pumping
Impact of Goreisan components on rat mesenteric collecting lymphatic vessel pumping
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DOI：
10.1111/micc.12788
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发表时间：
2022-10-06
期刊：
影响因子：
2.4
通讯作者：
Breslin，Jerome W.
中科院分区：
文献类型：
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作者：
Jo，Michiko;Trujillo，Andrea N.;Breslin，Jerome W.
研究背景Goreisan是一种传统的具有利尿作用的草药制剂，在日本被用作缓解水肿的临床治疗药物。本研究旨在确定Goreisan及其五种不同成分如何影响淋巴泵功能。MethodsMesenteric收集淋巴管从麻醉的Sprague-Dawley大鼠中分离，并安装在37°C生理盐水浴中的电阻匹配的玻璃微量移液器上进行研究。连续测量直径以获得以下淋巴泵参数：收缩频率（CF）、舒张末期直径（EDD）和收缩末期直径（ESD）、收缩幅度（AMP）、射血分数（EF）和泵流量分数（FPF）。Goreisan和它的每一个组成部分（黄柏，黄芪，泽泻，多孔菌，和黄芪）被施加到浴中的浓度为1-30 μg/mL.ResultsThe结果表明，虽然Goreisan引起淋巴泵没有显着变化，泽泻和多孔菌各自显着降低CF和FPF。此外，大鼠口服给药Goreisan和泽泻1周后，其肠系膜集合淋巴管中VEGFR-3的表达升高。ConclusionsCollectively，结果表明，Goreisan的某些成分对淋巴泵有直接，快速的影响。这些发现提供了新的见解，但也提出了关于Goreisan在继发性水肿患者中的治疗潜力的新问题。
BackgroundGoreisan is a traditional herbal formulation with diuretic properties tested as a clinical therapeutic to alleviate lymphedema in Japan. The present study aimed to determine how Goreisan and its five different components affect lymphatic pump function.MethodsMesenteric collecting lymphatics were isolated from anesthetized Sprague–Dawley rats and mounted on resistance‐matched glass micropipettes in a 37°C physiological salt solution bath for studies. Diameter was continuously measured to obtain the following lymphatic pump parameters: contraction frequency (CF), end diastolic diameter (EDD), and end systolic diameter (ESD), contraction amplitude (AMP), ejection fraction (EF), and fractional pump flow (FPF). Goreisan and each of its components (Cinnamomi Cortex, Atractylodis Rhizoma, Alismatis Rhizoma, Polyporus, and Poria) were applied to the bath at concentrations of 1–30 μg/mL.ResultsThe results show that while Goreisan causes no significant changes to lymphatic pumping, Alismatis Rhizoma and Polyporus each significantly reduce CF and FPF. In addition, rats that received oral administration of Goreisan and Alismatis Rhizoma for 1 week had elevated expression of VEGFR‐3 in their mesenteric collecting lymphatics.ConclusionsCollectively, the results suggest that some components of Goreisan have a direct, rapid impact on lymphatic pumping. These findings provide new insights but also raise new questions about the therapeutic potential of Goreisan in patients with secondary lymphedema.