Human mononuclear leukocyte chemotaxis: a quantitative assay for humoral and cellular chemotactic factors.
Human mononuclear leukocyte chemotaxis: a quantitative assay for humoral and cellular chemotactic factors.
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人单核白细胞趋化性：体液和细胞趋化因子的定量测定。
DOI：
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发表时间：
1972
影响因子：
4.4
通讯作者：
S. E. Mergenhagen
中科院分区：
文献类型：
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作者：
R. Snyderman;Leonard C. Altman;Marvin S. Hausman;S. E. Mergenhagen
单核白细胞（MNL）在伤口愈合和宿主免疫中的参与是有充分记录的（1,2）。尽管如此，这些细胞在局部组织部位积聚的机制尚不清楚。最近的研究表明，来自兔或豚鼠炎症渗出液的mnl可以在体外对各种刺激产生化学反应（3-6；M. S. Hausman， R. Snyderman和S. E. Mergenhagen，已提交发表）。然而，人类MNL趋化活性的定量研究方法尚未见报道。对人类MNL趋化性的测定可以更清楚地定义人类MNL趋化因子，并评估MNL在各种人类疾病状态下的功能。本报告描述了一种可重复的定量分析，用于人类起源的体液和细胞因子，这些因子对人类MNLs具有趋化作用。
The participation of the mononuclear leukocyte (MNL) in wound healing and in host immunity is well documented (1, 2). Despite this, the mechanism by which these cells accumulate in local tissue sites is poorly defined. Recent studies have shown that MNLs derived from rabbit or guinea pig inflammatory exudates can respond chemotactically to various stimuli in vitro (3–6; M. S. Hausman, R. Snyderman and S. E. Mergenhagen, submitted for publication). However, quantitative methods for the study of human MNL chemotactic activity have not been previously reported. An assay of human MNL chemotaxis would allow a clearer definition of human MNL chemotactic factors and also the assessment of MNL function in various human disease states. The present report describes a reproducible, quantitative assay for humoral and cellular factors of human origin which are chemotactic for human MNLs.
Materials and Methods. Source of MNLs.