A study of side reactions occurring during synthesis of oligodeoxynucleotides containing O6-alkyldeoxyguanosine residues at preselected sites.
A study of side reactions occurring during synthesis of oligodeoxynucleotides containing O6-alkyldeoxyguanosine residues at preselected sites.
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研究在预选位点合成含有 O6-烷基脱氧鸟苷残基的寡脱氧核苷酸过程中发生的副反应。
作者：
H. Borowy‐Borowski;R. Chambers
作为致癌物致突变分子机制研究的一部分，我们合成了12个在预选位置含有O6-烷基鸟嘌呤残基的寡核苷酸(15-MERS)，用于酶法合成具有生物活性的DNA。这些寡核苷酸中有10个来自负链序列，该负链序列携带噬菌体Phi x174 DNA的基因G的第三密码子中的修饰核苷酸。另外两个来自携带人类Ha-ras原癌基因第12密码子修饰的正链序列。在这项工作中，观察到了几个潜在的严重副反应，这可能会使突变数据的解释复杂化。本文对这些反应进行了详细的研究。由于我们无法避免不受欢迎的副产品，我们开发了简单的层析方法来检测和去除它们。
As part of our studies on the molecular mechanisms of mutation by carcinogens we have synthesized 12 oligonucleotides (15-mers) containing an O6-alkylguanine residue at a preselected position for use as primers in the enzymatic synthesis of biologically active DNA. Ten of these oligonucleotides are derived from a minus strand sequence carrying the modified nucleotide in the third codon of gene G of bacteriophage phi X174 DNA. Two others are derived from plus strand sequences carrying the modification in the 12th codon of the human Ha-ras protooncogene. During this work several potentially serious side reactions, which could complicate interpretation of mutagenesis data, were observed. This paper describes a detailed study of these reactions. Since we were unable to avoid undesirable side products, we developed simple chromatographic methods for detecting and removing them.
特异烷基化的 DNA 片段。
影响因子：
14.9
作者：
Kuzmich,S;Marky,LA;Jones,RA
通讯作者：
Jones,RA
O6-甲基鸟嘌呤突变和修复是不均匀的。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Topal,MD;Eadie,JS;Conrad,M
通讯作者：
Conrad,M