The optic lobe-pars intercerebralis axis is involved in circa’bi’dian rhythm of the large black chafer Holotrichia parallela
The optic lobe-pars intercerebralis axis is involved in circa’bi’dian rhythm of the large black chafer Holotrichia parallela
复制标题
视叶-脑间轴参与大黑金龟子 Holotrichia parala 的昼夜节律
DOI：
10.1007/s00359-020-01440-8
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Shiga Sakiko
中科院分区：
文献类型：
--
作者：
Watanabe Kohei;Shiga Sakiko
大黑金龟子Holotrichia parallela48小时左右的节律。虽然昼夜节律被认为与生物钟有关，但还没有进行生理学研究来验证这种参与。我们研究了视叶或大脑间部切除对昼夜节律的影响。切除双侧视叶后，所有甲虫均失去昼夜节律（N= 25），但切除单侧视叶后，所有甲虫均表现出昼夜节律（N= 18）。然而，后一组中有22%的人表现出白天转换。切除大脑间部后，26.3%的甲虫表现出了寄生模式（N= 19）。三聚乙醛品红染色的大脑间部细胞的数量在脑缺血组显着减少相比，在完整的和假手术组。大脑间部切除的甲虫的活性显著高于对照组。结果表明，视叶和至少部分大脑白质是昼夜节律所必需的，而双侧视叶是维持H中两日节律规则性所必需的。这表明，神经回路的昼夜节律钟细胞在视叶到pars lateralis可能是进化保守的，也用于产生昼夜节律。
The large black chaferHolotrichia parallelaexhibits ~ 48-h circa’bi’dian rhythm. Although circabidian rhythm is suggested to involve the circadian clock, no physiological studies have been conducted to verify this involvement. We examined the effects of optic lobe or pars intercerebralis removal on the circabidian rhythm. After removing both optic lobes, all beetles lost their circabidian rhythms (N= 25), but all beetles exhibited circabidian rhythm after removing unilateral optic lobe (N= 18). However, 22% of the latter group exhibited day switching. After removal of the pars intercerebralis, 26.3% beetles showed arrhythmic patterns (N= 19). The number of paraldehyde fuchsin-stained pars intercerebralis cells in the arrhythmic group was significantly reduced compared to in the intact and sham-operated groups. The activity in the pars intercerebralis-removed beetles was significantly higher than that in the control groups. The results show that the optic lobe and at least part of the pars intercerebralis are necessary for circabidian rhythm, and bilateral optic lobes are necessary to maintain regularity of the two-day rhythm inH. parallela.This suggests that a neural circuit of circadian clock cells in the optic lobe to pars lateralis might be evolutionally conserved and used also for the generation of circabidian rhythm.