Structural analysis of N-glycans from gull egg white glycoproteins and egg yolk IgG.
Structural analysis of N-glycans from gull egg white glycoproteins and egg yolk IgG.
复制标题
海鸥蛋清糖蛋白和蛋黄 IgG 的 N-聚糖的结构分析。
DOI：
10.1093/glycob/cwp025
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发表时间：
2009
期刊：
影响因子：
4.3
通讯作者：
Lee,YuanC
中科院分区：
文献类型：
--
作者：
Suzuki,Noriko;Su,Tseng-Hsiung;Wu,Sze-Wei;Yamamoto,Kazuo;Khoo,Kay-Hooi;Lee,YuanC
我们以前的研究表明，（Galα1-4Gal）糖蛋白在鸟类中的表达与它们的生殖发育有关。然而，仅已知鸽蛋白色糖蛋白和IgG中含有（Galα1-4Gal）的N-聚糖的精确结构。为了比较其他物种含（Galα1- 4 Gal）N-聚糖的结构特征，我们采用高效液相色谱（HPLC）、质谱（MS）和质谱/质谱（MS/MS）分析了海鸥卵白色（GEW）-糖蛋白、卵类粘蛋白、卵转铁蛋白和海鸥卵黄IgG的N-聚糖。GEW糖蛋白包括中性、单唾液酸和双唾液酸N-聚糖，其中一些含有Galα1-4Gal序列。缺乏二等分GlcNAc的双触角、三触角和四触角寡糖是主要的核心结构，不完全的α-半乳糖基化和唾液酸化以及分支上存在的diLacNAc产生了N-聚糖结构的微观异质性。此外，与鸽子蛋白色糖蛋白不同，GEW糖蛋白中的主要唾液酸化是α 2，3-连接的唾液酸（NeuAc），而不是α 2，6-连接的唾液酸（NeuAc）。除了复合型低聚糖外，缺乏二等分GlcNAc的混合型低聚糖在GEW-糖蛋白中也很丰富。海鸥卵黄IgG也含有Galα1-4Galβ1-4GlcNAcβ1-序列，但与鸽IgG不同，未检测到Galα1- 4Gal β 1 - 4Gal β 1 -4GlcNAcβ1-序列。具有二分GlcNAc和核心岩藻糖基化的双触角和三触角复合型低聚糖以及高甘露糖型低聚糖是海鸥IgG的主要结构。我们的数据表明，GEW糖蛋白和海鸥IgG中的一些N-聚糖含有Galα1-4Galβ1-4GlcNAcβ1-序列，但N-聚糖非还原末端的α-Gal帽残基与非α-Gal帽残基的比例远低于鸽糖蛋白。
We previously showed that the expression of (Galα1-4Gal)-bearing glycoproteins among birds is related to their phylogeny. However, precise structures of (Galα1-4Gal)-containingN-glycans were only known for pigeon egg white glycoproteins and IgG. To compare structural features of (Galα1-4Gal)-containingN-glycans from other species, we analyzedN-glycans of gull egg white (GEW)-glycoproteins, ovomucoid, and ovotransferrin, and gull egg yolk IgG by HPLC, mass spectrometry (MS), and MS/MS analyses. GEW-glycoproteins included neutral, monosialyl, and disialylN-glycans, and some of them contained Galα1-4Gal sequences. Bi-, tri-, and tetra-antennary oligosaccharides that lacked bisecting GlcNAc were the major core structures, and incomplete α-galactosylation and sialylation as well as the presence of diLacNAc on the branches generated microheterogeneity of theN-glycan structures. Moreover, unlike pigeon egg white glycoproteins, the major sialylation in GEW-glycoproteins is α2,3-, but not α2,6-linked sialic acids (NeuAc). In addition to the complex-type oligosaccharide, hybrid-type oligosaccharides that lack bisecting GlcNAc were also abundant in GEW-glycoproteins. Gull egg yolk IgG also contained Galα1-4Galβ1-4GlcNAcβ1- sequences, but unlike pigeon IgG, no Galα1-4Galβ1-4Galβ1-4GlcNAcβ1- sequence was detected. Bi- and tri-antennary complex-type oligosaccharides with bisecting GlcNAc and with core fucosylation as well as high-mannose-type oligosaccharides were the major structures in gull IgG. Our data indicated that someN-glycans from both GEW-glycoproteins and gull IgG contain the Galα1-4Galβ1-4GlcNAcβ1- sequence, but the ratio of α-Gal-capped residues to non-α-Gal-capped residues in the nonreducing termini ofN-glycans is much lower than that in those of pigeon glycoproteins.