Na(+)/Ca(2+) exchanger contributes to stool transport in mice with experimental diarrhea.
Na(+)/Ca(2+) exchanger contributes to stool transport in mice with experimental diarrhea.
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DOI：
10.1292/jvms.16-0475
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发表时间：
2017-02-28
期刊：
影响因子：
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通讯作者：
Takeuchi T
中科院分区：
文献类型：
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作者：
Nishiyama K;Tanioka K;Azuma YT;Hayashi S;Fujimoto Y;Yoshida N;Kita S;Suzuki S;Nakajima H;Iwamoto T;Takeuchi T
Na+/Ca 2+交换器（NCX）是一种受膜电位和Na+和Ca 2+跨膜梯度控制的双向转运蛋白。为了揭示NCX对胃肠运动的功能作用，我们以前使用NCX 1和NCX 2杂合子敲除小鼠（HET）。我们发现，NCX 1和NCX 2在胃底、回肠和远端结肠的运动中起重要作用。因此，我们认为NCX 1和NCX 2在肠内容物的转运中起重要作用。在这里，我们研究了NCX在药物诱导的腹泻小鼠模型中的作用。采用硫酸镁、5-羟色胺（5-HT）和前列腺素E2（PGE 2）诱导的腹泻评价NCX 1 HET和NCX 2 HET中的粪便排泄物。NCX 2 HET，而不是NCX 1 HET，通过增加水样粪便而加剧硫酸镁诱导的腹泻。同样，5-HT诱导的充血在NCX 2 HET中加重，但在NCX 1 HET中未加重。然而，NCX 1 HET和NCX 2 HET显示PGE 2诱导的腹泻与野生型小鼠（WT）的腹泻相似。与先前显示的远端结肠的结果一样，在WT的近端和横结肠中，肌间神经丛层以及纵向和环形肌肉层对NCX 1和NCX 2具有强烈的免疫反应性。在这项研究中，我们证明了NCX 2在腹泻的发展中具有重要作用。
The Na+/Ca2+ exchanger (NCX) is a bidirectional transporter that is controlled by membrane potential and transmembrane gradients of Na+ and Ca2+. To reveal the functional role of NCX on gastrointestinal motility, we have previously used NCX1 and NCX2 heterozygote knockout mice (HET). We found that NCX1 and NCX2 play important roles in the motility of the gastric fundus, ileum and distal colon. Therefore, we believed that NCX1 and NCX2 play an important role in transport of intestinal contents. Here, we investigated the role of NCX in a mouse model of drug-induced diarrhea. The fecal consistencies in NCX1 HET and NCX2 HET were assessed using a diarrhea induced by magnesium sulfate, 5-hydroxytryptamine (5-HT) and prostaglandin E2 (PGE2). NCX2 HET, but not NCX1 HET, exacerbated magnesium sulfate-induced diarrhea by increasing watery fecals. Likewise, 5-HT-induced diarrheas were exacerbated in NCX2 HET, but not NCX1 HET. However, NCX1 HET and NCX2 HET demonstrated PGE2 induced diarrhea similar to those of wild-type mice (WT). As well as the result of the distal colon shown previously, in the proximal and transverse colons of WT, the myenteric plexus layers and the longitudinal and circular muscle layers were strongly immunoreactive to NCX1 and NCX2. In this study, we demonstrate that NCX2 has important roles in development of diarrhea.