Inhibitory effect of glyceollin isolated from soybean against melanogenesis in B16 melanoma cells
Inhibitory effect of glyceollin isolated from soybean against melanogenesis in B16 melanoma cells
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DOI：
10.5483/bmbrep.2010.43.7.461
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发表时间：
2010-07-31
期刊：
影响因子：
3.8
通讯作者：
Lee, Young-Sup
中科院分区：
文献类型：
--
作者：
Lee, Young-Sang;Kim, Hyun-Kyoung;Lee, Young-Sup
与人工合成化学品相比，具有无毒和环保特性的天然产品是皮肤美白化妆品的良好资源。本文研究了大豆抗疫菌素对大豆黑素合成的影响，并探讨了大豆抗疫菌素在黑素合成中的作用机制。我们发现大豆抗毒素抑制816黑色素瘤细胞的黑色素合成和酪氨酸酶活性，而没有细胞毒性。为了阐明大豆抗素对黑素生成的作用机制，我们对黑素生成酶如酪氨酸酶、酪氨酸酶相关蛋白-1（TRP-1）和TRP-2进行了蛋白质印迹分析。Glyceollin抑制酪氨酸酶和TRP-1蛋白表达。此外，大豆抗毒素有效地抑制由α-黑素细胞刺激激素（α-MSH）刺激的B16黑素瘤细胞中的细胞内cAMP水平。这些结果表明，大豆抗毒素的美白活性可能是由于在B16黑色素瘤细胞中抑制参与α-MSH信号通路的cAMP。[BMB报告2010; 43（7）：461-467]
Natural products with non-toxic and environmentally friendly properties are good resources for skin-whitening cosmetic agents when compared to artificial synthetic chemicals. Here, we investigated the effect of glyceollin produced to induce disease resistance responses of soybean to specific races of an incompatible pathogen, phytophthora sojae, on melanogenesis and discussed their mechanisms in melanin biosynthesis. We found that glyceollin inhibits melanin synthesis and tyrosinase activity in 816 melanoma cells without cytotoxicity. To elucidate the mechanism of the effect of glyceollin on melanogenesis, we conducted western blot analysis for melanogenic enzymes such as tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2. Glyceollin inhibited tyrosinase and TRP-1 protein expression. Additionally, glyceollin effectively inhibited intracellular cAMP levels in B16 melanoma cells stimulated by alpha-melanocyte stimulating hormone (alpha-MSH). These results suggest that the whitening activity of glyceollin may be due to the inhibition of cAMP involved in the signal pathway of alpha-MSH in B16 melanoma cells. [BMB reports 2010; 43(7): 461-467]