DIFFERENTIAL SENSITIVITY OF MOUSE OOCYTES TO COLCHICINE-INDUCED ANEUPLOIDY
DIFFERENTIAL SENSITIVITY OF MOUSE OOCYTES TO COLCHICINE-INDUCED ANEUPLOIDY
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DOI：
10.1002/em.2850100208
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发表时间：
1987-01-01
影响因子：
2.8
通讯作者：
YUAN, ZP
中科院分区：
文献类型：
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作者：
MAILHES, JB;YUAN, ZP
我们实验室未发表的结果表明，当与人绒毛膜促性腺激素（HCG）同时注射秋水仙碱时，可增加小鼠中期II （MII）卵母细胞的发生率。本研究的目的是确定使用秋水仙碱的时间是否影响MII卵母细胞非整倍体的发生率。CD-1小鼠给予妊娠母马血清（PMS）， 48小时后给予HCG。腹腔注射0.2 mg/kg秋水仙碱，给予相对于HCG +4、+2、0、-2或-4小时。hcg后17小时收集卵母细胞并进行处理，染色体随后呈c带状。对照组、hcg前/后+4、+2、0、-2或-4小时的超倍体卵母细胞比例分别为0.77、2.56、5.17、7.79、3.54和2.70。这些数据的卡方分析表明，秋水仙碱显著增加了非整倍体卵母细胞的比例，并且秋水仙碱诱导的非整倍体的相对敏感性取决于该药物相对于HCG的使用时间。
Unpublished results from our laboratory showed that colchicine increased the incidence of hyperploid mouse metaphase II (MII) oocytes when injected at the same time as human chorionic gonadotropin (HCG). The objective of the present study was to determine whether the time of administering colchicine influenced the incidence of aneuploidy in MII oocytes. CD-1 mice were given pregnant mare''s serum (PMS) and, 48 hr later, HCG. An intraperitoneal injection of 0.2 mg/kg colchicine were given +4, +2, 0, -2, or -4 hr relative to HCG. Oocytes were collected 17 hr post-HCG and processed, and chromosomes were subsequently C-banded. The percentage of hyperploid oocytes was 0.77, 2.56, 5.17, 7.79, 3.54, and 2.70 for control, +4, +2, 0, -2, or -4 hr pre/post-HCG, respectively. Chi-square analyses of these data demonstrated that colchicine significantly increases the proportion of aneuploid oocytes, and that the relative sensitivity of colchicine-induced aneuploidy depends upon the time that this drug is administered relative to HCG.