Investigation of survival and migration potential of differentiated cardiomyocytes transplanted with decellularized heart scaffold
Investigation of survival and migration potential of differentiated cardiomyocytes transplanted with decellularized heart scaffold
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DOI：
10.1002/jbm.a.36572
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发表时间：
2019-03-01
影响因子：
4.9
通讯作者：
Onur, Mehmet Ali
中科院分区：
文献类型：
--
作者：
Akbay, Esin;Onur, Mehmet Ali
间充质干细胞衍生的心肌细胞被用作心肌细胞移植的来源以及用于脱细胞组织支架中的组织工程。本研究旨在研究分化的心肌细胞整合到脱细胞支架植入大鼠腹膜后的存活和迁移潜力，并评估其异位用于未来心血管组织工程的可行性。为此，首先分离脂肪组织来源的间充质干细胞（AdMSC）。用溴脱氧尿苷（BrdU）标记细胞。通过应用离子和非离子去污剂获得脱细胞心脏组织支架，并将标记的分化细胞接种到这些测试的脱细胞支架上。在植入1、2和4周后，通过各种技术检查无细胞支架（CFS）或细胞支架（CS）复合物的植入细胞的异位迁移潜力和支架上的接种细胞之间的相互作用。在植入的第一周和第二周，在肾组织样本中观察到阳性染色细胞。在第1、2和4周期间对心肌细胞特异性基因表达的观察显示，在每个时间段内潜力增加。逆转录聚合酶链反应（RT-PCR）结果显示，在支架上接种的细胞之间的相互作用增加，但CFS测试组的基因表达没有显着差异。(c)2018年威利期刊公司J Biomed Mater Res Part A：107A：561-570，2019.
Mesenchymal stem cell-derived cardiomyocytes are employed as a source for myocardial cell transplantation as well as for tissue engineering in decellularized tissue scaffolds. The present study aimed at investigating the survival and migration potential of differentiated cardiomyocytes integrated to decellularized scaffolds after implantation into retroperitoneum of rats, and to assess the feasibility of their ectopic use for future cardiovascular tissue engineering. For this purpose, adipose tissue-derived mesenchymal stem cells (AdMSCs) were first isolated. Cells were labeled by bromodeoxyuridine (BrdU). Decellularized cardiac tissue scaffolds were acquired by application of ionic and non-ionic detergents and the labeled differentiated cells were seeded onto these tested decellularized scaffolds. After 1, 2, and 4 weeks of implantation, either cell free scaffold (CFS) or cell scaffold (CS) composites were examined by various techniques for ectopic migration potential of the implanted cells and interaction between the seeded cells on scaffolds. Throughout the first and second weeks of implantation, positively stained cells were observed in renal tissue samples. Observations, for cardiomyocytes-specific gene expression during weeks 1, 2, and 4, showed potential increased over each time period. A reverse transcription polymerase chain reaction (RT-PCR) results revealed an increased interaction between cells seeded on scaffolds, however CFS test groups showed no significant difference in gene expression. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 561-570, 2019.