The human immunodeficiency virus type 1 Vpu protein enhances membrane permeability
The human immunodeficiency virus type 1 Vpu protein enhances membrane permeability
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DOI：
10.1021/bi981527f
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发表时间：
1998-09-29
期刊：
影响因子：
2.9
通讯作者：
Carrasco, L
中科院分区：
文献类型：
--
作者：
González, ME;Carrasco, L
T淋巴细胞被人类免疫缺陷病毒感染后，感染细胞的细胞内阳离子含量会发生剧烈变化。人类免疫缺陷病毒1型基因组编码几种辅助蛋白。包括VPU，一种在平面脂质双层中形成离子通道的完整膜蛋白。分析了VPU对质膜对几种分子通透性的影响。VPU在大肠杆菌中的表达增加了膜对一些分子的通透性，如2-硝基苯基-β-D-半乳糖苷、尿苷、不透性的翻译抑制剂潮霉素B和溶菌酶。此外，VPU在真核COS细胞中的瞬时表达增强了潮霉素B和神经生物素等带电分子进入这些细胞的能力。VPU对细胞膜通透性的影响与已报道的来自不同动物病毒的其他膜活性蛋白相似，包括M2型流感病毒、塞姆利基森林病毒6K和脊髓灰质炎病毒2B和3A蛋白。
Infection of T lymphocytes by the human immunodeficiency virus causes drastic alterations in the intracellular cation content of the infected cells. The human immunodeficiency virus type 1 genome encodes several accessory proteins. including Vpu, an integral membrane protein that forms ion channels in planar Lipid bilayers. The effect of Vpu on the permeability of the plasma membrane to several molecules has been analyzed. Expression of vpu in Escherichia coli cells increases membrane permeability to a number of molecules such as 2-nitrophenyl beta-D-galactopyranoside, uridine, the impermeable translation inhibitor hygromycin B, and lysozyme. In addition, transient expression of Vpu in eukaryotic COS cells enhances entry of charged molecules such as hygromycin B and neurobiotin into these cells. The effect of Vpu on cell membrane permeability resembles that reported for other membrane-active proteins from different animal viruses, including influenza M2, Semliki Forest virus 6K, and poliovirus 2B and 3A proteins.