ICAM-1 expression on endothelium and systemic cytokine production in cutaneous neutrophilic leukocytoclastic vasculitis in NZBxNZWF1 mice
ICAM-1 expression on endothelium and systemic cytokine production in cutaneous neutrophilic leukocytoclastic vasculitis in NZBxNZWF1 mice
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DOI：
10.14670/hh-20.45
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发表时间：
2005-01-01
影响因子：
2
通讯作者：
Ichinohe, N
中科院分区：
文献类型：
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作者：
Hayashi, T;Hasegawa, K;Ichinohe, N
本研究在人类系统性红斑狼疮模型NZBxNZWF（1）（B/WF 1）小鼠中检测了皮肤微血管损伤与细胞因子在内皮细胞上表达粘附分子之间的关系。在临床表现明显的老年患者中，中性粒细胞主要粘附于毛细血管和小静脉内皮，并伴有碎裂（白细胞破碎），导致血管损伤（白细胞破碎性血管炎），而在早期患者中则无此现象。这通过从血管中泄漏monstral blue得到证实。在此阶段，LFA-1（+）白细胞粘附于内皮上强烈表达的ICAM-1，这与循环中IL-1 α和TNF-α的显著升高有关。本研究提示，IL-1 α和TNF-α可能是B/WF 1小鼠皮肤微血管内皮细胞ICAM-1表达增加，导致以嗜中性白细胞破碎为特征的血管损伤的部分原因。
The present study has examined relationship between cutaneous microvessel injury and adhesion molecule expression on the endothelium by cytokines in NZBxNZWF(1) (B/WF1) mice, a model for human systemic lupus erythematosus. In advanced ages associated with overt clinical manifestation, but not in early ages, neutrophils with a minor proportion of monocytes and lymphocytes mainly adhered to the endothelium of capillary and the venule with fragmentation (leukocytoclasis), leading to the vascular injury (leukocytoclastic vasculitis). This was confirmed by the leak of monstral blue from the blood vessel. At this stage LFA-1(+) leukocytes adhered to intensely expressed ICAM-1 on the endothelium, and this was paralleled with a significant rise in IL-1alpha and TNF-alpha in the circulation. The present study suggests that IL-1alpha and TNF-a may, at least in part, be responsible for the increased ICAM-1 expression on endothelium in cutaneous microvessels, resulting in the vascular injury characterized by neutrophilic leukocytoclasis in B/WF1 mice.