A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system.
A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system.
复制标题
鸡肝细胞系可能通过其高水平病毒受体和高效蛋白质表达系统有效支持 ALV-J 的复制
DOI：
10.1186/s13567-018-0537-7
复制
发表时间：
2018-05-02
影响因子：
4.4
通讯作者：
Ye J
中科院分区：
文献类型：
--
作者：
Li T;Xie J;Lv L;Sun S;Dong X;Xie Q;Liang G;Xia C;Shao H;Qin A;Ye J
本研究中，我们鉴定了一个能够支持高滴度ALV-J（J亚群禽白血病病毒）复制的鸡肝细胞系（LMH）。值得注意的是，ALV-J在DF 1细胞前1天通过ELISA在LMH细胞中被有效地检测到。与DF 1细胞相比，LMH细胞不仅表达更高水平的ALV-J受体chNHE-1，而且具有更有效的外源基因蛋白表达系统。因此，LMH细胞可能是一种新的工具，以缩短ALV-J根除的方法，并加速研究的发病机制和肿瘤的ALV-J。
In this study, we identified a chicken liver cell line (LMH) which could strongly support the replication of ALV-J (Subgroup J of avian leukosis virus) with high viral titer. Notably, ALV-J was efficiently detected by ELISA in LMH cells 1 day before DF1 cells. In comparison with DF1 cells, LMH cells not only expressed higher levels of ALV-J receptor chNHE-1, but also possessed a more efficient protein expression system for foreign genes. Thus, LMH cells could be a novel tool to shorten the ALV-J eradication approach and accelerate studies on the pathogenesis and oncogenesis of ALV-J.