The heat shock response of Synechocystis sp. PCC 6803 analysed by transcriptomics and proteomics.
The heat shock response of Synechocystis sp. PCC 6803 analysed by transcriptomics and proteomics.
复制标题
DOI：
10.1093/jxb/erj148
复制
发表时间：
2006-04
影响因子：
6.9
通讯作者：
I. Suzuki;W. Simon;A. Slabas
中科院分区：
文献类型：
--
作者：
I. Suzuki;W. Simon;A. Slabas
当蓝细菌集胞藻属PCC 6803的细胞暴露于高温时，它们感知生长条件的变化并调节基因的表达和合成热诱导蛋白质作为对热应激的响应。DNA微阵列分析显示，伴侣蛋白和蛋白酶的基因，如groESL 1，groEL 2，htpG，hspA，和clpB1在44 ℃孵育细胞20分钟后瞬时诱导。双向凝胶电泳显示，这些伴侣蛋白和蛋白酶的水平在44 ℃孵育细胞60分钟后升高。在集胞藻细胞中，伴侣蛋白的mRNA和蛋白质的表达具有很好的相关性。然而，延伸因子的水平主要在蛋白质水平上调节。这些结果表明，集胞藻对热激条件的适应可能是由转录和翻译调控决定的。
When cells of the cyanobacterium Synechocystis sp. PCC 6803 are exposed to high temperature they perceive changes in the growth conditions and regulate the expression of genes and synthesize heat-inducible proteins as a response to the heat stress. DNA microarray analysis revealed that genes for chaperonins and proteases, such as groESL1, groEL2, htpG, hspA, and clpB1 were transiently induced after incubation of the cells at 44 degrees C for 20 min. Quantitative two-dimensional gel electrophoresis revealed that the levels of these chaperonins and proteases were elevated after incubation of cells at 44 degrees C for 60 min. These findings indicated that levels of the mRNAs and proteins of chaperonins were well correlated in the cells of Synechocystis. However, the level of elongation factors are mainly regulated at the protein level. These results indicated that acclimation to the heat-shock conditions might be governed by transcriptional and translational regulation in Synechocystis.