TRANSNEURONAL TRANSPORT OF WHEAT-GERM-AGGLUTININ CONJUGATED HORSERADISH-PEROXIDASE INTO LAST ORDER SPINAL INTERNEURONS PROJECTING TO ACROMIODELTOIDEUS AND SPINODELTOIDEUS MOTONEURONS IN THE CAT .2. DIFFERENTIAL LABELING OF INTERNEURONS DEPENDING ON MOVEMENT TYPE
TRANSNEURONAL TRANSPORT OF WHEAT-GERM-AGGLUTININ CONJUGATED HORSERADISH-PEROXIDASE INTO LAST ORDER SPINAL INTERNEURONS PROJECTING TO ACROMIODELTOIDEUS AND SPINODELTOIDEUS MOTONEURONS IN THE CAT .2. DIFFERENTIAL LABELING OF INTERNEURONS DEPENDING ON MOVEMENT TYPE
复制标题
DOI：
10.1007/bf00228851
复制
发表时间：
1990-01-01
影响因子：
2
通讯作者：
KUMMEL, H
中科院分区：
文献类型：
--
作者：
ALSTERMARK, B;KUMMEL, H
用麦胚凝集素标记的辣根过氧化物酶跨神经元运输来确定自愿到达靶点和/或无限制行走时最后一级脊髓间神经元投射三角肌运动神经元的位置。在到达靶点的猫中，从C2到Th1的两侧都发现了标记的神经元间神经元，而在行走的猫中，几乎只在C-5Th1节段发现了标记的神经元间神经元，尽管这些猫的标记神经元总数比靶向到达猫的要高得多。这些结果证实了先前的发现，即C3-C4节段的固有脊髓神经元可以通过前肢调节到达靶点的下行指令。两组猫同侧V-IX板层均有标记神经元，对侧主要局限于第VIII板层。行走猫前肢节段V-VII板外侧部及第VIII、IX板层标记神经元数目较多。在所有猫中，标记的神经元间神经元和运动神经元的总数之间几乎是线性的正相关。结果表明，兴奋性和抑制性末级神经元均可被跨神经元标记。结论：该方法可用于不同动作准备和/或执行过程中活跃的末级神经元的功能鉴定。
Transneuronal transport of wheat germ agglutinin conjugated horseradish peroxidase was used to define the location of last order spinal interneurones projecting the deltoideus motoneurones during voluntary target-reaching and/or in unrestricted walking on the group. Labelled interneurones were found bilaterally from C2 to Th1 in target-reaching cats and almost exclusively in the C-5Th1 segments in walking cats, although the total number of labelled interneurones in these cats was considerably higher than in the target-reaching cats. These results confirm the previous finding that propriospinal neurons in the C3-C4 segments can mediate the descending command for target-reaching movements with the forelimb. In both groups of cats labelled interneurones were found ipsilaterally in laminae V-IX, while contralaterally they were mainly restricted to lamina VIII. In the forelimb segments there was a larger number of labelled interneurones in the walking cats in the lateral part of laminae V-VII and the laminae VIII and IX. There was a positive, almost linear correlation between the total number of labelled interneurones and motoneurones in all cats. The results suggest that both excitatory and inhibitory last order interneurones can be transneuronally labelled. It is concluded that this method can be used for functional identification of last order interneurones active during the preparation and/or execution of different movements.