Brief anesthesia by isoflurane alters plasma corticosterone levels distinctly in male and female rats: Implications for tissue collection methods.
Brief anesthesia by isoflurane alters plasma corticosterone levels distinctly in male and female rats: Implications for tissue collection methods.
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DOI：
10.1016/j.bbr.2016.03.003
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发表时间：
2016-05-15
影响因子：
2.7
通讯作者：
Neigh GN
中科院分区：
文献类型：
--
作者：
Bekhbat M;Merrill L;Kelly SD;Lee VK;Neigh GN
麻醉剂的安乐死通常在采集组织之前进行，以尽量减少对动物的痛苦和痛苦。然而，根据其作用机制和给药方案的不同，不同的麻醉方法可能会通过接触下丘脑-垂体-肾上腺(HPA)轴而触发急性应激反应，这可能会影响许多其他生理过程，研究人员可能希望将其作为终点来检查。我们研究了常用麻醉剂异氟烷对雄性和雌性大鼠血浆皮质酮水平和糖皮质激素受体(GR)及其几种调节因子(包括FK506结合蛋白51(FKBP5))基因表达的影响。我们的结果表明，断头前短暂的异氟醚麻醉可以在几分钟内改变雄性和雌性大鼠的血浆皮质酮水平，而不会影响海马区的基因表达。我们的结论是，收集方法会影响雌性大鼠的应激相关生理终点，在实验设计过程和数据解释中，应该评估即使是短暂麻醉的潜在影响，以及麻醉反应的性别差异。鉴于美国国立卫生研究院关于性别和可再生性的新标准，这一发现尤其重要，应注意确保感兴趣终点的性别差异不是对收集范例的性别差异的产物。
Euthanasia by anesthetic agents is commonly performed prior to tissue collection in order to minimize pain and distress to the animal. However, depending on their mechanism of action as well as administration regimen, different methods of anesthesia may trigger an acute stress response through engaging the hypothalamic-pituitary-adrenal (HPA) axis, which can impact numerous other physiological processes that the researcher may wish to examine as endpoints. We investigated the effects of the commonly used anesthetic agent isoflurane on two different endpoints related to the stress response: plasma corticosterone levels and gene expression of the glucocorticoid receptor (GR) as well as several of its regulators including FK506-binding protein 51 (Fkbp5) in the hippocampus of male and female rats. Our results indicate that brief exposure to anesthesia by isoflurane prior to decapitation can alter plasma corticosterone levels differentially in male and female rats within minutes without impacting gene expression in the hippocampus. We conclude that collection methods can influence stress-related physiological endpoints in female rats and the potential influence of even brief anesthesia as well as sex differences in response to anesthesia should be evaluated during the experimental design process and data interpretation. This finding is particularly important in light of new NIH standards regarding sex and reproducibility, and care should be taken to be certain that sex differences in endpoints of interest are not an artifact of sex differences in response to collection paradigms.