Rheological properties of cell-hydrogel composites extruding through small-diameter tips
Rheological properties of cell-hydrogel composites extruding through small-diameter tips
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DOI：
10.1115/1.2896215
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发表时间：
2008-04-01
影响因子：
4
通讯作者：
Xiong, Zhuo
中科院分区：
文献类型：
--
作者：
Cheng, Jie;Lin, Feng;Xiong, Zhuo
利用活细胞和水凝胶的复合材料形成三维结构正成为生物工程和制造领域的一项有吸引力的技术。已经提出了不同的工艺，其中大部分是基于蜂窝复合挤压和沉积技术。因此，蜂窝挤出喷嘴的设计成为一个重要的阶段，而蜂窝-水凝胶复合材料的流变性能是一个关键的参数，必须进行深入的研究。本文研制了一种用于小直径喷嘴的入口压力检测装置和适配器。在幂律流体类型的假设下，计算了幂律常数和幂函数系数。实验结果表明，细胞-水凝胶复合材料(最大细胞浓度为10(7)/ml)在流变性能上与水凝胶材料没有差别。在此基础上，讨论了细胞-水凝胶复合材料的可制造性。此外，还对细胞组装技术中的另一个重要问题--细胞活力进行了讨论。当挤压速率为0.5 mm(3)/S时，细胞所占比例达到最大值(97%)，挤压时间和细胞承受的最大剪应力是两种影响机制。
To form 3D structures with composites of living cells and hydrogel is now becoming an attractive technology in both bioengineering and manufacturing areas. Variant processes have been presented, most of which are based on the cell composite extrusion and deposition technique. So, the design of cell extrusion nozzle turns into an important phase, and the rheological properties of cell-hydrogel composites act as a key parameter and must be well investigated. In this paper an entrance pressure detection device and adaptor for small-diameter tips were developed. With the hypothesis of power law fluid type, the power law constant and coefficient were calculated. As derived from the experimental results, the cell-hydrogel composites (with the maximum cell concentration of 10(7)/Ml) show no difference from hydrogel materials in rheological properties. Based on these, the manufacturability of cell-hydrogel composites was discussed. Also, the cell viability, which is another important issue in cell assembly technology, was discussed. The peak percentage (97%) was achieved at the flow rate of 0.5 mm(3)/s. The extruding duration and the maximum shear stress loaded on cells were considered as two influence mechanisms.