Cytokine profile of human adipose-derived stem cells: Expression of angiogenic, hematopoietic, and pro-inflammatory factors
Cytokine profile of human adipose-derived stem cells: Expression of angiogenic, hematopoietic, and pro-inflammatory factors
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DOI：
10.1002/jcp.21068
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发表时间：
2007-09-01
影响因子：
5.6
通讯作者：
Gimble, Jeffrey M.
中科院分区：
文献类型：
--
作者：
Kilroy, Gail E.;Foster, Sandra J.;Gimble, Jeffrey M.
脂肪组织是脂肪因子和细胞因子的来源，在健康和疾病中具有局部和全身作用。在这项研究中，我们检验了这样的假设：多能人类脂肪源性干细胞（ASC）能够沿着脂肪细胞、软骨细胞和成骨细胞途径分化，有助于脂肪组织源性细胞因子的分泌。暴露于碱性成纤维细胞生长因子 (bFGF) 或表皮生长因子 (EGF) 后，ASC 显着增加肝细胞生长因子 (HGF) 的分泌，肝细胞生长因子是一种与造血、血管生成和乳腺上皮导管形成有关的细胞因子。抗坏血酸与这些诱导因子协同作用，进一步提高 HGF 水平。根据 ELISA 和 RT-PCR，暴露于脂多糖后，ASC 会增加造血细胞（粒细胞/单核细胞、粒细胞和巨噬细胞集落刺激因子、白细胞介素 7）和促炎细胞因子（白细胞介素 6、8 和 11、肿瘤坏死因子 a）的分泌。在与脐带血来源的 CD34(+) 细胞建立的共培养物中，ASC 支持体外的长期造血作用。此外，在短期 12 天的共培养中，ASC 维持并扩大了骨髓和淋巴祖细胞的数量。这些观察结果与分泌细胞因子的功能一致，并证实了其他实验室最近关于 ASC 造血支持能力的报告。我们得出的结论是，ASC 表现出的细胞因子分泌特性与报道的骨髓间充质干细胞 (MSC) 相似。
Adipose tissue serves as a source of adipokines and cytokines with both local and systemic actions in health and disease. In this study, we examine the hypothesis that multipotent human adipose-derived stem cells (ASCs), capable of differentiating along the adipocyte, chondrocyte, and osteoblast pathways, contribute to adipose tissue-derived cytokine secretion. Following exposure to basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF), the ASCs significantly increase their secretion of hepatocyte growth factor (HGF), a cytokine implicated in hematopoiesis, vasculogenesis, and mammary epithelial duct formation. Ascorbic acid synergizes with these inductive factors, further increasing HGF levels. Following exposure to lipopolysaccharicle, ASCs increase their secretion of both hematopoietic (granulocyte/monocyte, granulocyte, and macrophage colony stimulating factors, interleukin 7) and proinflammatory (interleukins 6, 8, and 11, tumor necrosis factor a) cytokines based on ELISA and RT-PCR. In co-cultures established with umbilical cord blood-derived CD34(+) cells, the ASCs support long-term hematopoiesis in vitro. Furthermore, in short-term 12-day co-cultures, the ASC maintain and expand the numbers of both myeloid and lymphoid progenitors. These observations are consistent with the functionality of the secreted cytokines and confirm recent reports by other laboratories concerning the hematopoietic supportive capability of ASCs. We conclude that the ASCs display cytokine secretory properties similar to those reported for bone marrow-derived mesenchymal stem cells (MSCs).