Differences Between High- and Low-Motility Buffalo Sperm Identified by Comparative Proteomics
Differences Between High- and Low-Motility Buffalo Sperm Identified by Comparative Proteomics
复制标题
通过比较蛋白质组学鉴定高活力和低活力水牛精子之间的差异
DOI：
10.1111/rda.12511
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发表时间：
2015-06-01
影响因子：
1.7
通讯作者：
Zhang, M.
中科院分区：
文献类型：
--
作者：
Huang, Y-L;Fu, Q.;Zhang, M.
内容本研究旨在探讨沼泽布法罗高活力和低活力精子蛋白质表达的差异。该研究使用二维凝胶电泳（2DE）结合基质辅助激光解吸/电离飞行时间串联质谱（MALDI-TOF/TOF-MS）来分析不同的蛋白质。结果表明，在布法罗精子和低活力精子中存在18个差异表达蛋白点，其中8个蛋白在低活力精子中表达上调，5个蛋白表达下调，1个蛋白缺失，4个蛋白特异性表达。最后，通过MS成功鉴定了四种蛋白质，它们属于三种独特的蛋白质;它们是精子尾部外致密纤维蛋白2（ODF 2）、ATP合成酶亚基α（ATP 5A 1）和琥珀酰辅酶A合成酶亚基β（SUCLG 2）。总之，这些结果有助于发展与低活力精子相关的分子机制的理解，并为寻找与精子活力相关的分子标记物提供线索。
Contents This study was undertaken to investigate differences in protein expression between high- and low-motility sperm of swamp buffalo. The research used two-dimensional gel electrophoresis (2DE) coupled to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF-MS) to analyse the different proteins. The results showed 18 different expression protein spots between high- and low-motility buffalo sperm; eight of these proteins were up-regulated in low-motility sperm, five were down-regulated, one deleted and four proteins specifically expressed. Finally, four proteins were successfully identified by MS as belonging to three unique proteins; they are outer dense fibre of sperm tails protein 2 (ODF2), ATP synthase subunit alpha (ATP5A1) and succinyl-CoA synthetase subunit beta (SUCLG2). In summary, these results help to develop an understanding of the molecular mechanisms associated with low-motility sperm and provide clues for finding molecular markers associated with sperm motility.