The effect of alginate and hyaluronate on the viability and function of immunoisolated neonatal rat islets.
The effect of alginate and hyaluronate on the viability and function of immunoisolated neonatal rat islets.
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海藻酸盐和透明质酸盐对免疫分离的新生大鼠胰岛的活力和功能的影响。
DOI：
10.1016/s0142-9612(99)00119-2
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发表时间：
1999
期刊：
影响因子：
14
通讯作者：
J. Schrezenmeir
中科院分区：
文献类型：
--
作者：
F. Velten;C. Laue;J. Schrezenmeir
最近的观察表明，细胞外基质在朗格汉斯胰岛长期培养的活力和形态完整性方面起着重要作用。本研究用海藻酸盐溶液（AL）和透明质酸盐溶液（HY）将未加细胞外基质（WEM）的新生大鼠胰岛包埋在cuphane中空纤维中。第1周和第5周后进行功能测试。在培养期间，WEM包封胰岛的胰岛素释放明显减少。与此相反，AL和HY包埋胰岛在整个培养过程中分泌值稳定。组织学检查证实，HY和AL包埋的胰岛的活力与WEM包埋的胰岛有显著差异。此外，HY似乎是一个比AL更有利于免疫分离胰岛的环境。HY包埋胰岛的胰岛素分泌值和活力都高于AL包埋胰岛。我们得出结论，细胞外基质对于维持免疫分离胰岛的功能和形态完整性是重要的，而HY特别适合于这一应用。
Recent observations suggest that the extracellular matrix plays a role as regards the viability and morphological integrity in long-term culture of Langerhans islets. For the present study we encapsulated neonatal rat islets without extracellular matrix (WEM), with alginate solution (AL) and with hyaluronate solution (HY) into cuprophane hollow fibers. Function was tested after week 1 and 5. The insulin release of WEM encapsulated islets decreased significantly during the culture period. In contrast to this, AL and HY embedded islets had stable secretion values throughout the whole cultivation. Histological examination confirmed that viability of HY and of AL embedded islets differed significantly from that of WEM encapsulated islets. Furthermore, HY seems to be a more advantageous environment to immunoisolated islets than AL. Both the insulin secretion values and the viability of HY embedded islets were higher than of AL embedded islets. We conclude that an extracellular matrix is important for immunoisolated islets, to maintain their function and morphological integrity and that HY is especially suitable for this application.