Immunophenotypic characterization of human bone marrow mast cells. A flow cytometric study of normal and pathological bone marrow samples
Immunophenotypic characterization of human bone marrow mast cells. A flow cytometric study of normal and pathological bone marrow samples
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DOI：
10.1155/1998/341340
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发表时间：
1998-01-01
影响因子：
3.2
通讯作者：
San Miguel, JF
中科院分区：
文献类型：
--
作者：
Escribano, L;Orfao, A;San Miguel, JF
本研究的目的是用流式细胞仪分析单抗进行多重染色，确定正常人和恶性血液病患者骨髓肥大细胞(BMMC)的免疫表型。我们的结果表明，两组人的BMMC表现出相似但不同的免疫表型。HM组的BMMC总数较高(p=0.08)。检测到三种抗原表达模式：(1)在所有被分析的病例中持续阳性的标记(CD9、CD29、CD33、CD43、CD44、CD49d、CD49e、CD51、CD71、CD117和Fc epsilon RI)，(2)持续阴性的抗原(CD1a、CD2、CD3、CD5、CD6、CD11a、CD14、CD15、CD16、CD19、CD20、CD21、CD23、CD25、CD30、CD34、CD38、CD41a、CD42b、CD65、CD66b、CD6、CD11a、CD14、CD15、CD16、CD19、CD20、CD21、CD23、CD25、CD30、CD34、CD38、CD41a、CD42b、CD65、CD66b、HLA-DR和CD138)以及(3)在不同比例的病例中呈阳性的标记-CD11b(50%)、CD21、CD23、CD25、CD30、CD34、CD38、CD41a、CD42b、CD65、CD66b、HLA-DR和CD138CD11c(77%)、CD13(40%)、CD18(20%)、CD22(68%)、CD35(27%)、CD40(67%)、CD54(88%)和CD61(40%)。此外，所有病例的BMMC均为CD45(+)，且该抗原的表达强度与成熟粒细胞相似。综上所述，我们的结果表明，健康对照组和HM患者的BMMC表现出相对不同的免疫表型。有趣的是，我们观察到BMMC和其他组织的MC的免疫表型明显不同。这可能是由于人MC根据其组织定位的异质性，或者是由于用于抗原检测的方法的敏感性。
The goal of the present paper was to define the immunophenotype of bone marrow mast cells (BMMC) from healthy controls and patients with hematologic malignancies (HM) based on the use of multiple stainings with monoclonal antibodies analyzed by flow cytometry. Our results show that BMMC from both groups of individuals display a similar but heterogenous immunophenotype. The overall numbers of BMMC are higher in the HM group of individuals (p = 0.08). Three patterns of antigen expression were detected: (1) markers constantly positive in all cases analyzed (CD9, CD29, CD33, CD43, CD44, CD49d, CD49e, CD51, CD71, CD117, and Fc epsilon RI), (2) antigens that were constantly negative (CD1a, CD2, CD3, CD5, CD6, CD11a, CD14, CD15, CD16, CD19, CD20, CD21, CD23, CD25, CD30, CD34, CD38, CD41a, CD42b, CD65, CD66b, HLA-DR, and CD138), and (3) markers that were positive in a variable proportion of cases - CD11b (50%), CD11c (77%), CD13 (40%), CD18 (20%), CD22 (68%), CD35 (27%), CD40 (67%), CD54 (88%) and CD61 (40%). In addition, BMMC from all cases explored were CD45(+), and this antigen was expressed at an intensity similar to that of mature granulocytes.In summary, our results show that BMMC from both healthy controls and HM patients display a relatively heterogenous immunophenotype. Interestingly, we have observed clear differences between the immunophenotype of BMMC and MC from other tissues. This could be due either to the heterogeneity of human MC according to their tissue localization or to the sensitivity of the method used for antigen detection.