Heme oxygenase-1 acts as an antiviral factor for porcine reproductive and respiratory syndrome virus infection and over-expression inhibits virus replication in vitro
Heme oxygenase-1 acts as an antiviral factor for porcine reproductive and respiratory syndrome virus infection and over-expression inhibits virus replication in vitro
复制标题
血红素加氧酶-1作为猪繁殖与呼吸综合征病毒感染的抗病毒因子，过表达抑制病毒体外复制
DOI：
10.1016/j.antiviral.2014.07.011
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发表时间：
2014-10-01
影响因子：
7.6
通讯作者：
Zhou, En-Min
中科院分区：
文献类型：
--
作者：
Xiao, Shuqi;Zhang, Angke;Zhou, En-Min
病毒复制依赖于受感染细胞中的宿主细胞过程，这对于猪繁殖与呼吸综合征病毒（PRRS）是真实的，PRRS的病原体是对养猪业的全球性威胁。血红素加氧酶-1（HO-1）是血红素降解的第一和限速酶，是一种广泛表达的诱导型同工酶。我们前期的研究提示HO-1在PRRSV感染中可能起重要作用。然而HO-1在PRRSV感染中的作用尚不清楚。本研究以Marc-145、PK-15（CD 163）细胞系和猪肺泡巨噬细胞（PAM）为实验材料，研究HO-1的诱导和过表达对两种不同的PRRSV株复制的影响。HO-1的诱导显著降低了PRRSV株在不同细胞中的复制。同样，腺病毒介导的HO-1的过表达也大大降低了PRRSV的复制。相比之下，使用小干扰RNA消融HO-1伴随着增加PRRSV复制。因此，这些数据与HO-1作为抗病毒因子的作用是一致的，并且这些发现表明HO-1的过表达或诱导可能提供针对PRRSV感染的潜在治疗策略。(C)2014爱思唯尔有限公司版权所有。
Virus replication depends upon host-cell processes in infected cells, and this is true for porcine reproductive and respiratory syndrome virus (PRRSV), the causative agent of PRRS that is a worldwide threat to the swine industry. Heme oxygenase-1 (HO-1) is a ubiquitously expressed inducible isoform of the first and rate-limiting enzyme for heme degradation. Our previous research suggested that HO-1 may play an important role in PRRSV infection. However, the function of HO-1 in PRRSV infection is unclear. In the present study, Marc-145, PK-15(CD163) cell lines and porcine alveolar macrophages (PAMs) were used to evaluate the effects of HO-1 induction and over-expression on the replication of two different PRRSV strains. Induction of HO-1 markedly decreased the replication of PRRSV strains in the different cells. Similarly, adenoviral-mediated over-expression of HO-1 also greatly decreased the replication of PRRSV. In contrast, ablation of HO-1 using small interfering RNA concomitantly increased PRRSV replication. Therefore, the data were consistent with HO-1 acting as an antiviral factor and these findings suggested that over-expression or induction of HO-1 may provide a potential therapeutic strategy against PRRSV infection. (C) 2014 Elsevier B.V. All rights reserved.