In vivo tracing of triazole pesticides in Chinese cabbage via a novel solid-phase microextraction fiber
In vivo tracing of triazole pesticides in Chinese cabbage via a novel solid-phase microextraction fiber
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DOI：
10.1016/j.foodcont.2023.110143
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发表时间：
2023-10
期刊：
影响因子：
6
通讯作者：
Yongming Duan;Yiquan Huang;Liling Huang;Zhangmin Xiang;Jian Liu;Shuqin Liu;Zhuo Chen
中科院分区：
文献类型：
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作者：
Yongming Duan;Yiquan Huang;Liling Huang;Zhangmin Xiang;Jian Liu;Shuqin Liu;Zhuo Chen
本研究利用席夫碱机理制备了富氮三嗪基多孔聚合物（NTP）。NTP含氮量高（35.47%），BET比表面积大，吸附平均孔径分别为860 m2g−1和3.92 nm。此外，NTP中的仲胺（- c - nh -）和三嗪氮（- cdouble bondN -）提供了大量的氮活性位点，有利于三唑类农药的吸附。将合成的NTP作为固相微萃取（SPME）萃取相材料制备NTP-SPME纤维，其对三唑类杀菌剂的提取性能显著高于商用纤维（p< 0.05）。随后，通过活体spme取样和气相色谱四极杆飞行时间质谱法对三种三唑类杀菌剂（myclobutanil、propiconazole和difenoconazole）在活蔬菜中的生物积累和消除进行了跟踪研究。结果表明，菌丁尼、丙环康唑和异虫康唑的浓度在第一天最高，在生物积累过程中呈先降低后平衡，在消除过程中逐渐降低的趋势。白菜茎中菌丁尼、丙环康唑和异虫康唑的最高浓度分别为997.57±51.42、841.20±21.42和56.82±1.30 ng/g。此外，在整个试验期间，异虫康唑的浓度远低于其他农药，这可能与它的强疏水性和低溶解度使其难以被白菜根部吸收有关。本研究重点利用NTP-SPME技术对三唑类杀菌剂在大白菜中的应用情况进行跟踪，为三唑类杀菌剂的合理使用及生态风险评价提供有价值的信息。
In this study, a nitrogen-rich triazine-based porous polymer (NTP) was prepared by the Schiff base mechanism. The NTP contains a high nitrogen content (35.47%) with a larger BET surface area and an adsorption average pore diameter of 860 m2g−1and 3.92 nm, respectively. In addition, secondary amines (–C–NH–) and triazine nitrogen (–Cdouble bondN–) in NTP provide a large number of nitrogen active sites, which facilitate the adsorption of triazole pesticides. The synthesized NTP was then used as the solid phase microextraction (SPME) extraction phase material to fabricate the NTP-SPME fiber, which had significantly higher performance in extracting triazole fungicides compared to the commercial fibers (p< 0.05). Subsequently, the bioaccumulation and elimination of three triazole fungicides (myclobutanil, propiconazole, and difenoconazole) in living vegetables were tracked byin vivoSPME sampling and quantified using a gas chromatography quadrupole time-of-flight mass spectrometry. The results showed that the concentrations of myclobutanil, propiconazole and difenoconazole were highest on the first day and showed a trend of first decreasing then equilibrium during bioaccumulation and gradually decreasing during elimination. The highest concentrations of myclobutanil, propiconazole, and difenoconazole in cabbage stems were 997.57 ± 51.42, 841.20 ± 21.42, and 56.82 ± 1.30 ng/g, respectively. In addition, concentrations of difenoconazole were much lower than those of other pesticides throughout the experimental period, which may be related to the difficulty of uptake by cabbage roots due to its strong hydrophobicity and low solubility. This study highlights the application of NTP-SPME for tracking triazole fungicides in living Chinese cabbage plants, which provides valuable information to rationale use of triazole fungicides and their ecological risk assessment.