Gene-specific acquisition of hormonal responsiveness in rat liver during development.
Gene-specific acquisition of hormonal responsiveness in rat liver during development.
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发育过程中大鼠肝脏激素反应性的基因特异性获得。
DOI：
10.1002/jcb.240370211
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发表时间：
1988
影响因子：
4
通讯作者：
Kenney,FT
中科院分区：
文献类型：
--
作者：
Johnson,AC;Lee,KL;Isham,KR;Kenney,FT
克隆的cDNA被用于杂交分析，以评估晚期胎鼠肝脏中两个类似调节基因的激素反应。酪氨酸转氨酶基因和基因33的转录(Lee等人：J Biol Chem 260：16433-16438,1985年)在成人肝脏中被糖皮质激素和通常具有拮抗作用的激素剂胰岛素和cAMP各自促进，并且这两个基因的转录在出生时或出生前发育激活。研究发现，在宫内处理的胎儿肝脏中，每种激素调节剂都显著增加了基因33的mRNA。近乎沉默的转氨酶基因在胎肝中的表达被cAMP显著增强，但不能被糖皮质激素或胰岛素控制，该基因对胰岛素的反应能力直到产后几天才意识到。这些数据表明，个体基因对激素调节剂做出反应的能力在发育过程中具有特异性。
Cloned cDNAs were used in hybridization analyses to assess hormonal responsiveness of two similarly regulated genes in livers of late-term fetal rats. Transcription of the tyrosine aminotransferase gene and of gene 33 (Lee et al.: J Biol Chem 260: 16433–16438, 1985) is enhanced by glucocorticoids and by each of the usually antagonistic hormonal agents, insulin and cAMP, in adult liver, and that of both genes is developmentally activated at or just prior to birth. The mRNA of gene 33 was found to be significantly increased by each of the hormonal regulators in livers of fetuses treated in utero. Expression of the nearly silent aminotransferase gene in fetal liver was appreciably increased by cAMP but was refractory to control by either glucocorticoids or insulin; capacity of this gene to respond to insulin was not realized until several days postpartum. The data indicate specificity in the developmental acquisition of the capacity of individual genes to respond to hormonal regulators.