Comparative genomics and phylogenetic analysis of S-dysenteriae subgroup
Comparative genomics and phylogenetic analysis of S-dysenteriae subgroup
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DOI：
10.1360/062004-96
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发表时间：
2005-08-01
期刊：
影响因子：
--
通讯作者：
Qi, J
中科院分区：
文献类型：
--
作者：
Yang, E;Bin, W;Qi, J
通过使用含有大肠杆菌 K12 菌株 MG1655 的全基因组 ORF（开放阅读框，ORF）和痢疾杆菌 A1 菌株 Sd51197 的特定 ORF 的微阵列进行比较基因组杂交（CGH），研究了 13 种痢疾沙门氏菌血清型代表的基因组组成。 CGH结果表明，该血清型的基因组含有2654个源自大肠杆菌的保守ORF。然而，大肠杆菌的219个内在基因，包括原噬菌体基因、分子伴侣、特异性O抗原的合成等却缺失。此外，还通过水平转移获得了一些特定的基因，如II型分泌系统相关成分、铁转运相关基因等。根据基于遗传组成的系统发育树，证明A1、A2、A8、A10与其他痢疾沙门氏菌血清型不同。我们在本报告中的结果可能为痢疾杆菌的生理过程、致病性和进化提供新的见解。
Genomic compositions of representatives of thirteen S. dysenteriae serotypes were investigated by performing comparative genomic hybridization (CGH) with microarray containing the whole genomic ORFs (open reading frames, ORFs) of E. coli K12 strain MG1655 and specific ORFs of S. dysenteriae A1 strain Sd51197. The CGH results indicated the genomes of the serotypes contain 2654 conserved ORFs originating from E coli. However, 219 intrinsic genes of E. coli including those prophage genes, molecular chaperones, synthesis of specific O antigen and so on were absent. Moreover, some specific genes such as type II secretion system associated components, iron transport related genes and some others as well were acquired through horizontal transfer. According to phylogenic trees based on genetic composition, it was demonstrated that A1, A2, A8, A10 were distinct from the other S. dysenteriae serotypes. Our results in this report may provide new insights into the physiological process, pathogenicity and evolution of S. dysenteriae.