Downregulation of Pgc-1α expression by tea leaves and their by-products
Downregulation of Pgc-1α expression by tea leaves and their by-products
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茶叶及其副产品下调 Pgc-1α 表达
DOI：
10.1002/cbf.3006
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发表时间：
2014
影响因子：
3.6
通讯作者：
Funaba M
中科院分区：
文献类型：
--
作者：
Shibuya E;Murakami M;Kondo M;Kamei Y;Tomonaga S;Matsui T;Funaba M
先前的研究表明，肌肉Pgc‐1α的表达控制着肌纤维类型的比例。作为饲粮通过Pgc - 1α表达调控肌肉纤维类型比例的第一步，本研究探讨了饲料对Pgc - 1α表达的调节作用。制备了一个基于荧光素酶的Pgc‐1α报告基因构建体（Pgc‐1α（‐2553）‐luc），包含小鼠Pgc‐1α启动子（−2553 ~ +78 bp）。对33种饲料乙醇提取物的筛选表明，乌龙茶和烘培绿茶提取物可降低C2C12成肌细胞中Pgc‐1α（‐2553）‐luc的表达。茶叶提取物对Pgc‐1α的转录抑制作用在HepG2细胞中重现。我们进一步研究了茶渣酒精提取物及其青贮对Pgc‐1α转录的影响；茶渣青贮提取物抑制Pgc‐1α转录。用生茶叶、茶渣和茶渣青贮提取物处理可有效降低肌卫星细胞肌肉形成过程中Pgc‐1α mRNA水平。目前的研究结果表明，茶叶及其副产品可以用来调节肌肉纤维类型的比例。版权所有©2013 John Wiley & Sons， Ltd
Previous studies indicate that muscle Pgc‐1α expression governs the proportion of muscle fibre types. As a first step in using diet to manipulate the proportion of muscle fibre types by using Pgc‐1α expression, the present study investigates the modulation of Pgc‐1α expression by feedstuffs. A luciferase‐based Pgc‐1α reporter construct (Pgc‐1α(‐2553)‐luc) that contains the mouse Pgc‐1α promoter (−2553 to +78 bp) was prepared. A screen of ethanol extracts from 33 feedstuffs indicated that oolong tea and roasted green tea extracts decreased Pgc‐1α(‐2553)‐luc expression in C2C12 myoblasts. The transcriptional repression of Pgc‐1α by tea leaf extracts was reproduced in hepatic HepG2 cells. We further examined the effects of the alcohol extracts of tea waste and its silage on Pgc‐1α transcription; the tea waste silage extract inhibited Pgc‐1α transcription. Treatment with the extracts of raw tea leaves, tea waste and tea waste silage effectively decreased Pgc‐1α mRNA levels during myogenesis of myosatellite cells. The present results suggest that tea leaves and their by‐products could be used to modulate proportions of muscle fibre types. Copyright © 2013 John Wiley & Sons, Ltd.