Systemic inflammatory changes and increased oxidative stress in rural Indian women cooking with biomass fuels
Systemic inflammatory changes and increased oxidative stress in rural Indian women cooking with biomass fuels
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DOI：
10.1016/j.taap.2012.04.004
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发表时间：
2012-06-15
影响因子：
3.8
通讯作者：
Banerjee, Anirban
中科院分区：
文献类型：
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作者：
Dutta, Anindita;Ray, Manas Ranjan;Banerjee, Anirban
这项研究是为了调查与使用液化石油气(LPG)等更清洁的燃料相比，经常使用生物质烹饪是否会加剧全身炎症和氧化应激，从而可能导致印度农村妇女患心血管疾病(CVD)的风险增加。共有635名使用生物质烹饪的女性(中位年龄36岁)和452名年龄匹配的使用液化石油气烹饪的对照组女性参加了研究。采用双抗体夹心法检测血清白介素6(IL-6)、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白介素8(IL-8)。用流式细胞仪检测白细胞产生的活性氧(ROS)，用分光光度法检测红细胞超氧化物歧化酶(SOD)的活性。高血压是在联合委员会第七次报告之后诊断出来的。心动过速以每分钟100次的脉搏频率为单位。采用实时气溶胶监测仪对烹调区域内直径小于10微米和2.5微米的颗粒物(PM10和PM2.5)进行了测量。与对照组相比，生物质使用者室内空气中的颗粒物污染更多，血清中IL-6、IL-8、TNF-α和CRP水平显著升高，ROS生成增加37%，而SOD消耗减少41.5%，高血压和心动过速的患病率高于使用LPG的邻居。PM10和PM2.5水平与炎症、氧化应激和高血压的标志物呈正相关。炎症标志物与血压升高相关。使用生物质烹饪会加剧使用生物质燃料烹饪的贫穷妇女的全身炎症、氧化应激、高血压和心动过速，因此，与对照相比，她们容易患上心血管疾病。全身炎症和氧化应激可能是CVD发生发展的机制因素。(C)2012 Elsevier Inc.保留所有权利。
The study was undertaken to investigate whether regular cooking with biomass aggravates systemic inflammation and oxidative stress that might result in increase in the risk of developing cardiovascular disease (CVD) in rural Indian women compared to cooking with a cleaner fuel like liquefied petroleum gas (LPG). A total of 635 women (median age 36 years) who cooked with biomass and 452 age-matched control women who cooked with LPG were enrolled. Serum interleukin-6 (IL-6), C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8) were measured by ELISA. Generation of reactive oxygen species (ROS) by leukocytes was measured by flow cytometry, and erythrocytic superoxide dismutase (SOD) was measured by spectrophotometry. Hypertension was diagnosed following the Seventh Report of the Joint Committee. Tachycardia was determined as pulse rate >100 beats per minute. Particulate matter of diameter less than 10 and 2.5 mu m (PM10 and PM2.5, respectively) in cooking areas was measured using real-time aerosol monitor. Compared with control, biomass users had more particulate pollution in indoor air, their serum contained significantly elevated levels of IL-6, IL-8, TNF-alpha and CRP, and ROS generation was increased by 37% while SOD was depleted by 41.5%, greater prevalence of hypertension and tachycardia compared to their LPG-using neighbors. PM10 and PM2.5 levels were positively associated with markers of inflammation, oxidative stress and hypertension. Inflammatory markers correlated with raised blood pressure. Cooking with biomass exacerbates systemic inflammation, oxidative stress, hypertension and tachycardia in poor women cooking with biomass fuel and hence, predisposes them to increased risk of CVD development compared to the controls. Systemic inflammation and oxidative stress may be the mechanistic factors involved in the development of CVD. (C) 2012 Elsevier Inc. All rights reserved.