Spatiotemporal expression of four isoforms of Ca2+/calmodulin-dependent protein kinase I in brain and its possible roles in hippocampal dendritic growth
Spatiotemporal expression of four isoforms of Ca2+/calmodulin-dependent protein kinase I in brain and its possible roles in hippocampal dendritic growth
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DOI：
10.1016/j.neures.2006.09.013
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Kondo, Hisatake
中科院分区：
文献类型：
--
作者：
Kamata, Akifumi;Sakagami, Hiroyuki;Kondo, Hisatake
在多功能Ca 2 +/钙调蛋白依赖性蛋白激酶（CaMK）中，已显示CaMKI包含由在成熟脑中具有丰富表达的单独基因编码的四种结构相关同种型（α、β、γ和δ）的家族。在这项研究中，我们首先研究了发育基因表达的四种异构体的CaMKI在小鼠大脑中，特别注意海马结构的原位杂交分析。发现CaMKI的四种亚型在神经元发育过程中表现出不同的时空表达。我们还研究了在培养的海马神经元的树突形成的功能参与的CaMKI。CaMKI激酶死亡突变体的过表达降低了转染神经元的平均树突长度，而对初级树突的数量和分支指数没有任何显着影响。我们目前的研究结果提供了详细的解剖学信息的发育表达的四种异构体的CaMKI在小鼠大脑中，这代表了可能的功能参与的CaMKI在海马神经元的基底树突状生长。(c)2006年Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
Among multifunctional Ca2+/calmodulin-dependent protein kinases (CaMKs), CaMKI has been shown to comprise a family of four structurally related isoforms (alpha, beta, gamma, and delta) encoded by separate genes with abundant expression in mature brain. In this study, we first examined the developmental gene expression of the four isoforms of CaMKI in mouse brain with special attention to the hippocampal formation by in situ hybridization analysis. The four isoforms of CaMKI were found to exhibit distinct spatiotemporal expression during neuronal development. We also examined the functional involvement of CaMKI in the dendritic formation of cultured hippocampal neurons. The overexpression of kinase-dead mutants of CaMKI reduced the average dendritic length of the transfected neurons without any significant effects on the number of primary dendrites and the branching index. Our present findings provide the detailed anatomical information on the developmental expression of the four isoforms of CaMKI in mouse brain, which represents the possible functional involvement of CaMKI in the basal dendritic growth of hippocampal neurons. (c) 2006 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.