Evaluation of Vitamin D Standardization Program protocols for standardizing serum 25-hydroxyvitamin D data: a case study of the program's potential for national nutrition and health surveys
Evaluation of Vitamin D Standardization Program protocols for standardizing serum 25-hydroxyvitamin D data: a case study of the program's potential for national nutrition and health surveys
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DOI：
10.3945/ajcn.112.057182
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发表时间：
2013-06-01
影响因子：
7.1
通讯作者：
Sempos, Christopher T.
中科院分区：
文献类型：
--
作者：
Cashman, Kevin D.;Kiely, Mairead;Sempos, Christopher T.
背景资料：维生素D标准化计划（VDSP）制定了国家健康/营养调查中25-羟基维生素D [25（OH）D]测量程序的标准化协议，以促进25（OH）D测量准确且随时间、地点和实验室程序可比，从而改善公共卫生实践。目的：我们将VDSP方案应用于来自爱尔兰国家成人营养调查（NANS）的现有ELISA衍生血清25（OH）D数据作为病例研究调查，并通过比较方案评估其有效性。设计：将VDSP参考系统和方案应用于来自代表性NANS样本（n = 1118）的基于ELISA的血清25（OH）D数据。采用标准化LC-tandem MS对99份储存的血清进行再分析，并得出回归方程，得到预测的标准化血清25（OH）D值，然后将其与所有血清的LC-tandem MS再分析值进行比较。
Background: The Vitamin D Standardization Program (VDSP) has developed protocols for standardizing procedures of 25-hydroxyvitamin D [25(OH)D] measurement in National Health/Nutrition Surveys to promote 25(OH)D measurements that are accurate and comparable over time, location, and laboratory procedure to improve public health practice.Objective: We applied VDSP protocols to existing ELISA-derived serum 25(OH)D data from the Irish National Adult Nutrition Survey (NANS) as a case-study survey and evaluated their effectiveness by comparison of the protocol-projected estimates with those from a reanalysis of survey serums by using liquid chromatography-tandem mass spectrometry (LC-tandem MS).Design: The VDSP reference system and protocols were applied to ELISA-based serum 25(OH)D data from the representative NANS sample (n = 1118). A reanalysis of 99 stored serums by using standardized LC-tandem MS and resulting regression equations yielded predicted standardized serum 25(OH)D values, which were then compared with LC-tandem MS reanalyzed values for all serums.Results: Year-round prevalence rates for serum 25(OH)D concentrations