Myoblasts transplanted into rat infarcted myocardium are functionally isolated from their host
Myoblasts transplanted into rat infarcted myocardium are functionally isolated from their host
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DOI：
10.1073/pnas.1232447100
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发表时间：
2003-06-24
影响因子：
11.1
通讯作者：
Charpak, S
中科院分区：
文献类型：
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作者：
Léobon, B;Garcin, I;Charpak, S
移植到梗塞心肌中的生肌细胞的存活和分化使细胞移植成为心血管疾病新疗法的希望。该方法得到了骨骼肌成肌细胞移植的进一步支持，该移植被证明可以改善几种动物物种的心脏功能。尽管成肌细胞移植及其最近的人体试验取得了成功，但功能改善的机制仍不清楚。在这里，我们使用细胞内记录结合视频和荧光显微镜来确定用增强型 GFP 进行基因标记并移植到大鼠梗塞心肌中的成肌细胞是否保留兴奋和收缩特性，并积极参与心脏功能。我们的结果表明，移植的成肌细胞分化成特殊的超兴奋肌管，其收缩活性完全独立于邻近的心肌细胞。我们得出的结论是，移植细胞和宿主细胞之间的机电耦合以外的机制也参与了心脏功能的改善。
Survival and differentiation of myogenic cells grafted into infarcted myocardium have raised the hope that cell transplantation becomes a new therapy for cardiovascular diseases. The approach was further supported by transplantation of skeletal myoblasts, which was shown to improve cardiac performance in several animal species. Despite the success of myoblast transplantation and its recent trial in human, the mechanism responsible for the functional improvement remains unclear. Here, we used intracellular recordings coupled to video and fluorescence microscopy to establish whether myoblasts, genetically labeled with enhanced GFP and transplanted into rat infarcted myocardium, retain excitable and contractile properties, and participate actively to cardiac function. Our results indicate that grafted myoblasts differentiate into peculiar hyperexcitable myotubes with a contractile activity fully independent of neighboring cardiomyocytes. We conclude that mechanisms other than electromechanical coupling between grafted and host cells are involved in the improvement of cardiac function.